The blue light receptor Phototropin 1 suppresses lateral root growth by controlling cell elongation

We investigated the relationship between the blue light receptor phototropin 1 (phot1) and lateral root growth in Arabidopsis thaliana seedlings. Fluorescence and confocal microscopy images, as well as PHOT1 mRNA expression studies provide evidence that it is highly expressed in the elongation zone...

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Published inPlant biology (Stuttgart, Germany) Vol. 17; no. 1; pp. 34 - 40
Main Authors Moni, A., Lee, A.-Y., Briggs, W. R., Han, I.-S.
Format Journal Article
LanguageEnglish
Published England Blackwell Publishing Ltd 01.01.2015
Subjects
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - physiology
Arabidopsis - radiation effects
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Auxin
Biological Transport - drug effects
cell elongation
development
Gene Expression Regulation, Plant
Genes, Reporter
Hypocotyl - genetics
Hypocotyl - growth & development
Hypocotyl - physiology
Hypocotyl - radiation effects
Indoleacetic Acids - metabolism
lateral root
Light
Phenotype
Phosphoproteins - genetics
Phosphoproteins - metabolism
phototropin
Phototropism
Phthalimides - pharmacology
Plant Growth Regulators - metabolism
Plant Roots - genetics
Plant Roots - growth & development
Plant Roots - physiology
Plant Roots - radiation effects
Plants, Genetically Modified
Recombinant Fusion Proteins
Seedlings - genetics
Seedlings - growth & development
Seedlings - physiology
Seedlings - radiation effects
cell elongation
development
Auxin
lateral root
phototropin
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Summary:We investigated the relationship between the blue light receptor phototropin 1 (phot1) and lateral root growth in Arabidopsis thaliana seedlings. Fluorescence and confocal microscopy images, as well as PHOT1 mRNA expression studies provide evidence that it is highly expressed in the elongation zone of lateral roots where auxin is accumulating. However, treatment with the auxin transport inhibitor N-1-naphthylphthalamic acid significantly reduced PHOT1 expression in this zone. In addition, PHOT1 expression was higher in darkness than in light. The total number of lateral roots was higher in the phot1 mutant than in wild-type Arabidopsis. Cells in the elongation zone of lateral roots of the phot1 mutant were longer than those of wild-type lateral roots. These findings suggest that PHOT1 plays a role(s) in elongation of lateral roots through the control of an auxin-related signalling pathway.
Bibliography:ArticleID:PLB12187
ark:/67375/WNG-DBTN3NZP-Z
Ministry of Education, Science and Technology - No. 2009-0072217
Figure S1. Schematic representations of nucleotide sequence of the proximal promoter region of the phot1 gene. (A) Nucleotide sequence of the proximal promoter of phot1. The numbering of the sequence is relative to the transcription start site (+1) indicated by the arrow. The translation start site (ATG) is boxed. DNA binding sites of the Auxin Responsive Element (ARE), as predicted from MatInspector software (Genomatix), are indicated as AuxRE. (B) Comparison of AuxRE in phot1 promoter with other auxin-regulated gene (DR5 and ER7) promoters (Guilfoyle et al. ).
istex:3B3F545E6F246B7209D17F4A1C9B7511B901B285
NSF - No. 0843617
General Researcher Supporting Fund
National Research Foundation of Korea
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1435-8603
1438-8677
DOI:10.1111/plb.12187