Determination of the β-blocker atenolol in plasma by capillary zone electrophoresis

• Published in: Journal of Chromatography A Vol. 916; no. 1; pp. 297 - 304
• Main Authors: Arias, Rosalı́a, Jiménez, Rosa Mª., Alonso, Rosa Mª., Télez, Mercedes, Arrieta, Isabel, Flores, Piedad, Ortiz-Lastra, Eduardo
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 04.05.2001; Elsevier
• Subjects: Adrenergic beta-Antagonists - blood; Adult; Aged; Analysis; Atenolol; Atenolol - blood; Beta-blockers; Biological and medical sciences; Calibration; Electrophoresis, Capillary - methods; Female; General pharmacology; Humans; Male; Medical sciences; Middle Aged; Pharmacology. Drug treatments; Reproducibility of Results; Atenolol; Beta-blockers; Human; Hypertension; Solid phase extraction; Biological fluid; Aminoalcohol; Chemical analysis; Zone electrophoresis; Phase composition; Capillary electrophoresis; Cardiovascular disease; Patient; Mobile phase; Blood; Blood plasma; Ultraviolet detector; Hydroxyether; Sample preparation; β1-Adrenergic receptor; Antihypertensive agent; Antagonist; Quantitative analysis; Beta blocking agent
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(01)00564-7

A capillary zone electrophoretic method was optimised for the determination of the β-blocker atenolol in plasma. Separation was performed in an uncoated silica capillary of 58.5 cm (effective length 50 cm)×75 μm I.D., and detection was at 194 nm. The effects of the buffer (concentration and pH), the injection time, the voltage applied and the plasma clean-up procedure were studied. The determination of atenolol was achieved in less than 3 min, using an electrolyte of 50 m M H 3BO 3–50 m M Na 2B 4O 7 (50:50, v/v) pH 9, injected hydrodynamically for 4 s at 50 mbar and applying a voltage of +25 kV. This method was applied to the determination of atenolol in plasma of nine hypertensive patients (male and female, aged from 39 to 73 years). Atenolol concentrations found vary from 30 to 585 ng/ml.