CacyBP/SIP promotes tumor progression by regulating apoptosis and arresting the cell cycle in osteosarcoma

Osteosarcoma (OS) is the most common primary malignant bone tumor in pediatric and adolescent patients. The calcyclin-binding protein/Siah-1-interacting protein (CacyBP/SIP) performs an essential function in cell proliferation and apoptosis. The present study investigated the effect of CacyBP/SIP in...

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Published inExperimental and therapeutic medicine Vol. 20; no. 2; pp. 1397 - 1404
Main Authors Zhao, Ming, Zhang, Run-Zi, Qi, Dian-Wen, Chen, Hong-Yi, Zhang, Guo-Chuan
Format Journal Article
LanguageEnglish
Published Athens Spandidos Publications 01.08.2020
Spandidos Publications UK Ltd
D.A. Spandidos
Subjects
Antibodies
Apoptosis
Bone cancer
Cell cycle
Cell growth
Cyclin-dependent kinases
Experiments
Infection
Kinases
Osteosarcoma
Penicillin G
Protein binding
Proteins
RNA
Sarcoma
Scientific equipment industry
Software
Tumors
United States
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Summary:Osteosarcoma (OS) is the most common primary malignant bone tumor in pediatric and adolescent patients. The calcyclin-binding protein/Siah-1-interacting protein (CacyBP/SIP) performs an essential function in cell proliferation and apoptosis. The present study investigated the effect of CacyBP/SIP in OS cell proliferation and apoptosis. CacyBP/SIP mRNA expression levels were evaluated in four OS cell lines by quantitative PCR. CacyBP/SIP expression was downregulated in Saos-2 cells using a lentivirus transfection system and the transfection efficiency was analyzed. The effects of CacyBP/SIP downregulation on Saos-2 cell proliferation and colony-formation ability were evaluated by MTT and colony-formation assays. The effect of CacyBP/SIP knockdown on Saos-2 cell cycle and apoptosis was analyzed by flow cytometry cell sorting. The Cancer Genome Atlas (TCGA) data was analyzed for validation. Human OS cell lines Saos-2, MG-63, HOS and U20S expressed CacyBP/SIP mRNA. CacyBP/SIP knockdown significantly inhibited cell proliferation and colony-formation ability. Gj/S phase arrest was induced by CacyBP/SIP downregulation, which also resulted in the downregulation of CDK and cyclins and the upregulation of p21. In addition, CacyBP/SIP downregulation induced Saos-2 cell apoptosis mediated by Bax and Bcl-2. High expression of CacyBP/SIP was significantly associated with poor prognosis in TCGA sarcoma database. Thus, CacyBP/SIP performs important functions in the proliferation and apoptosis of human OS cells. Key words: osteosarcoma, proliferation, cell cycle, apoptosis
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ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2020.8843