MALDI mass spectrometry imaging discloses the decline of sulfoglycosphingolipid and glycerophosphoinositol species in the brain regions related to cognition in a mouse model of Alzheimer's disease

• Published in: Talanta (Oxford) Vol. 266; no. Pt 2; p. 125022
• Main Authors: Zhang, Qi, Li, Yan, Sui, Ping, Sun, Xue-Heng, Gao, Yufei, Wang, Chun-Yan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.01.2024
• Subjects: Alzheimer's disease; Brain lipids homeostasis; Cognition-related brain regions; MALDI-MSI; Transgenic mouse; Transgenic mouse; MALDI-MSI; Alzheimer's disease; Cognition-related brain regions; Brain lipids homeostasis
• ISSN: 0039-9140; 1873-3573; 1873-3573
• DOI: 10.1016/j.talanta.2023.125022

Aging and neurodegenerative disease are accompanied by lipid perturbations in the brain. Understanding the changes in the contents and functional activity of lipids remains a challenge not only because of the many areas in which lipids perform bioactivities but also because of the technical limitations in identifying lipids and their metabolites. In the present study, we aimed to evaluate how brain lipids are altered in Alzheimer's disease (AD)-like pathology by using mass spectrometry imaging (MSI). The spatial distributions and relative abundances of lipids in the brains were compared between APP/PS1 mice and their age-matched wild-type (WT) mice by matrix-assisted laser desorption ionization (MALDI) MSI assays. The comparisons were correlated with the analysis using a spectrophotometric method to determine the relative contents of sulfatides in different brain regions. Significant changes of brain lipids between APP/PS1 and WT mice were identified: eight sulfoglycosphingolipid species, namely, sulfatides/sulfated hexosyl ceramides (ShexCer) and two glycerophosphoinositol (GroPIn) species, PI 36:4 and PI 38:4. The declines in the spatial distributions of these ShexCer and GroPIn species in the APP/PS1 mice brains were associated with learning- and memory-related brain regions. Compared with young WT mice, aged WT mice showed significant decreases in the levels of these ShexCer and GroPIn species. Our results provide technical clues for assessing the impact of brain lipid metabolism on the senescent and neurodegenerative brain. The decline in sulfatides and GroPIns may be crucial markers during brain senescence and AD pathology. Appropriate lipid complementation might be important potentials as a therapeutic strategy for AD. [Display omitted] •The brain-lipid changes in an AD mouse model can be visualized by MALDI-MSI assays.•The declines of SHexCer and GroPIn species were identified in APP/PS1 mice brains.•The reduction of SHexCer and GroPIn species may be related to cognition impairment.•The decreases of SHexCer and GroPIn species may be involved in brain senescence.