A Novel Mouse Skin Graft Model of Vascular Tumors Driven by Akt1

To investigate whether endothelial Akt1 activation is sufficient to induce vascular tumor formation in the skin, we have developed a skin graft model in which a skin fragment from transgenic donor mice with inducible and endothelial cell-specific overexpression of activated Akt1 (myrAkt1) is grafted...

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Bibliographic Details
Published inBio-protocol Vol. 7; no. 13; p. e2369
Main Authors Phung, Thuy L, Ayyaswamy, Sriram
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 05.07.2017
Bio-protocol LLC
Subjects
Biology
Methods
Animal model
Vascular tumors
Hemangioma
Akt
Skin graft
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Summary:To investigate whether endothelial Akt1 activation is sufficient to induce vascular tumor formation in the skin, we have developed a skin graft model in which a skin fragment from transgenic donor mice with inducible and endothelial cell-specific overexpression of activated Akt1 (myrAkt1) is grafted into the skin of wild type recipient mice. The donor skin successfully engrafts after two weeks and, more importantly, vascular tumor develops at the site of transgenic skin graft when myrAkt1 expression is turned on. This skin graft model is a novel approach to investigate the biological impact of a key signal transduction molecule in a temporal, localized and organ-specific manner.
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ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2369