Differential gene expression in tumor adjacent histologically normal prostatic tissue indicates field cancerization

Field cancerization denotes the occurrence of aberrant cells in tumor adjacent histologically normal tissues (TAHN). To characterize field cancerization in prostate cancer, we used RNA from paired patient tumor and TAHN tissues excised at 1 cm from the tumor margin and subjected them to microarray e...

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Bibliographic Details
Published inInternational journal of oncology Vol. 35; no. 3; pp. 537 - 546
Main Authors HAALAND, C. M, HEAPHY, C. M, BUTLER, K. S, FISCHER, E. G, GRIFFITH, J. K, BISOFFI, M
Format Journal Article
LanguageEnglish
Published Athens Editorial Academy of the International Journal of Oncology 01.09.2009
Subjects
Aged
Biological and medical sciences
Gene Expression
Gene Expression Profiling
Humans
Male
Medical sciences
Middle Aged
Oligonucleotide Array Sequence Analysis
Prostate - pathology
Prostatic Neoplasms - genetics
Prostatic Neoplasms - pathology
Reverse Transcriptase Polymerase Chain Reaction
Tumors
tumor adjacent histologically normal
Cancerology
Tumoral tissue
Tumor
Gene expression
Urogenital system
Prostate
Field cancerization
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Summary:Field cancerization denotes the occurrence of aberrant cells in tumor adjacent histologically normal tissues (TAHN). To characterize field cancerization in prostate cancer, we used RNA from paired patient tumor and TAHN tissues excised at 1 cm from the tumor margin and subjected them to microarray expression analysis comparative to RNA from normal cancer-free prostatic tissues. Eleven novel transcripts were significantly up-regulated in TAHN tissues and also in tumors. Expression of early growth response protein 1, tristetraprolin, testican, and fatty acid synthase, mutually up-regulated at different levels in tumors and TAHN tissues was confirmed by quantitative reverse transcriptase PCR in the experimental and in an independent validation set. This study offers proof of expressional changes in field cancerized prostatic TAHN tissues at defined distances from tumor margins. Markers of field cancerized prostatic tissues could be early diagnostic indicators in biopsies after abnormal prostate-specific antigen and digital rectal examination and independent of cancerous histology and/or early targets for chemo-preventive intervention in pre-malignant disease.
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ISSN:1019-6439
1791-2423
DOI:10.3892/ijo_00000365