Evaluation of Toll-like receptor expression profile in patients with psoriasis vulgaris

• Published in: Gene Vol. 702; pp. 166 - 170
• Main Authors: Gürel, Gülhan, Sabah-Özcan, Seda
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 20.06.2019
• Subjects: acne; Adult; Female; Gene Expression; Humans; leprosy; Leukocytes, Mononuclear - metabolism; Male; messenger RNA; Middle Aged; mononuclear leukocytes; pathophysiology; patients; PBMC; Psoriasis; Psoriasis - blood; Psoriasis - genetics; Psoriasis - metabolism; quantitative polymerase chain reaction; TLRs, mRNA expression; Toll-Like Receptor 1 - blood; Toll-Like Receptor 1 - genetics; Toll-Like Receptor 10 - blood; Toll-Like Receptor 10 - genetics; Toll-Like Receptor 8 - blood; Toll-Like Receptor 8 - genetics; Toll-like receptors; Toll-Like Receptors - blood; Toll-Like Receptors - genetics; Toll-Like Receptors - metabolism; PASI; Psoriasis; TLRs, mRNA expression; mRNA; cDNA; qPCR; TLR; PBMC
• ISSN: 0378-1119; 1879-0038; 1879-0038
• DOI: 10.1016/j.gene.2019.03.058

TLRs are thought to play a role in the pathophysiology of such dermatological diseases as leprosy, acne and psoriasis. The study included 20 patients with plaque psoriasis, as well as 20 healthy age- and gender-matched control subjects. Real-time polymerase chain reaction evaluation was made of the messenger RNA expression of TLRs 1–10 in lesional tissue and peripheral blood mononuclear cell samples in psoriasis patients. TLR 3, 5, 6, 7, 9 and 10 lesional tissue mRNA expressions were increased significantly when compared to the expression levels in the PBMCs of the same patients (p = 0.0082, p = 0.0176, p = 0.0239, p = 0.0261, p = 0.0223, p = 0.0206). A comparison of the TLR expression in the PBMCs of healthy subjects and the PBMCs of patients with psoriasis showed a significant increase in the TLR 1, 8 and 10 mRNA expressions in the patient group (p < 0.0001, p < 0.0001, p = 0.0035). The TLR 5 mRNA expression was significantly higher in the control group than in the patient group (p = 0.0037). To the best of our knowledge, this is the first study in literature to evaluate mRNA TLR expression levels in the lesional tissue and PBMCs of patients with psoriasis. •TLR expression was higher in lesion tissue samples compared to PBMCs of the same psoriasis patients.•TLR 1, 8 and 10 mRNA expressions was higher in PBMCs of the patient with psoriasis than control group.•TLRs may play a role in the pathogenesis of psoriasis.