Automated normal-phase preparative high-performance liquid chromatography as a substitute for flash chromatography in the synthetic research laboratory

An automated normal-phase preparative HPLC system was developed in order to omit time-consuming flash column chromatography in the synthetic research laboratory. The system is equipped with steel columns packed with spherical 12 μm silica and is able to separate samples in a range of 0.1–10 g depend...

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Bibliographic Details
Published inJournal of Chromatography A Vol. 908; no. 1; pp. 143 - 148
Main Authors Renold, Peter, Madero, Elisio, Maetzke, Thomas
Format Journal Article Conference Proceeding
LanguageEnglish
Published Amsterdam Elsevier B.V 26.01.2001
Elsevier
Subjects
Analytical chemistry
Automation
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - methods
Chromatography, Thin Layer
Exact sciences and technology
Other chromatographic methods
Automation
Preparative chromatography
Separation method
Elution
Gradient
Multicomponent mixture
HPLC chromatography
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Summary:An automated normal-phase preparative HPLC system was developed in order to omit time-consuming flash column chromatography in the synthetic research laboratory. The system is equipped with steel columns packed with spherical 12 μm silica and is able to separate samples in a range of 0.1–10 g depending on the column diameter and chromatographic problem. It was designed to be used as an open access instrument in the research department. The general users select from binary gradient programs after running an analytical TLC with the raw product. The HPLC instrument was fully controlled by the Chromeleon software from Dionex. A Gilson 215 robot served as injector/collector.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-9673
DOI:10.1016/S0021-9673(00)00940-7