Membranous osteogenesis system modeled with KUSA-A1 mature osteoblasts

• Published in: Biochimica et biophysica acta Vol. 1725; no. 1; pp. 57 - 63
• Main Authors: Matsumoto, Satoshi, Shibuya, Isao, Kusakari, Satoshi, Segawa, Kaoru, Uyama, Taro, Shimada, Akinori, Umezawa, Akihiro
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30.08.2005
• Subjects: Animals; Calcification, Physiologic; Cell Differentiation; Cell Line; Cell Shape; Cells, Cultured; Female; Gap junction; Gap Junctions - physiology; KUSA; Membranous osteogenesis; Mice; Microscopy, Electron; Models, Biological; Osteoblast; Osteoblasts - cytology; Osteoblasts - physiology; Osteoblasts - ultrastructure; Osteogenesis - physiology; Stromal cell; Stromal Cells - cytology; Stromal Cells - metabolism; Osteoblast; Stromal cell; KUSA; Gap junction; Membranous osteogenesis
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/j.bbagen.2005.05.027

Several stromal cells were established from murine bone marrow cultures. One of the KUSA subclones, KUSA-A1 cells, displays osteogenic characteristics in vitro and in vivo. The calcium deposition, osteocalcin release, and parathyroid hormone (PTH) responsiveness of KUSA-A1 cells indicate that they are mature osteoblasts or osteocytes. Bone had formed in subcutaneous tissue 1 week after subcutaneous injection of cells into immunodeficient mice. The osteogenesis by KUSA-A1 was not mediated by chondrogenesis and thus was considered to be membranous ossification. These unique characteristics of KUSA-A1 cells provide an opportunity to analyze the process of membranous ossification in detail.