Isotachophoretic determination of naproxen in the presence of its metabolite in human serum

• Published in: Journal of Chromatography A Vol. 916; no. 1; pp. 207 - 214
• Main Authors: CAKRT, Miroslav, HERCEGOVA, Andrea, LESKO, Jan, POLONSKY, Jozef, SADECKA, Jana, SKACANI, Ivan
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 04.05.2001; Elsevier
• Subjects: Analysis; Anti-Inflammatory Agents, Non-Steroidal - blood; Biological and medical sciences; Calibration; Desmethylnaproxen; Electrophoresis - methods; General pharmacology; Humans; Hydrogen-Ion Concentration; Medical sciences; Naproxen; Naproxen - blood; Pharmacology. Drug treatments; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Fluorescence; Desmethylnaproxen; Naproxen; Isotachophoresis; Human; Biological fluid; Chemical analysis; Metabolite; Blood; Electrochemical detector; Non steroidal antiinflammatory agent; Analgesic; Conductometry; Antipyretic; Arylpropionic acid derivatives; Blood serum; Quantitative analysis
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(00)01071-2

An isotachophoretic method with conductivity detection was developed to determine naproxen in the presence of its metabolite 6- O-desmethylnaproxen in human serum. The leading electrolyte contained 10 m M hydrochloric acid, β-alanine, pH 4.0 and 0.1% methylhydroxypropylcellulose. The terminating electrolyte was 10 m M 2-( N-morpholino)ethanesulfonic acid–tris(hydroxymethyl)aminomethane, pH 6.9, containing 20% (v/v) of ethanol. Naproxen was determined in serum supernatant after simple deproteination of the sample with ethanol. The isotachophoretic results were compared with those obtained by synchronous fluorescence spectrometry.