4-(5,6-Dimethoxy-2-phthalimidinyl)phenylsulfonyl semipiperazide as a fluorescent labelling reagent for determination of carboxylic acids in precolumn high-performance liquid chromatography

• Published in: Journal of Chromatography A Vol. 816; no. 2; pp. 137 - 143
• Main Authors: Inoue, Hirofumi, Ikeno, Masaki, Ishii, Yuka, Tsuruta, Yasuto
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 14.08.1998; Elsevier
• Subjects: 4-(5,6-Dimethoxy-2-phthalimidinyl)phenylsulfonyl semipiperazide; Analytical chemistry; Biological and medical sciences; Carboxylic acids; Carboxylic Acids - chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid; Exact sciences and technology; Fatty acids; Fatty Acids - blood; Fluorescent Dyes - chemical synthesis; Fluorescent Dyes - pharmacology; Humans; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Miscellaneous. Technology; Other chromatographic methods; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Phthalimides - chemical synthesis; Phthalimides - pharmacology; Solvents; Spectrometry, Fluorescence; Detection, LC; Derivatization, LC; Carboxylic acids; 4-(5,6-Dimethoxy-2-phthalimidinyl)phenylsulfonyl semipiperazide; Fatty acids; Human; Biological fluid; Fluorescence detector; Chemical analysis; Phase composition; Aliphatic compound; Precolumn; HPLC chromatography; Derivatization; Experimental study; Mobile phase; Isocratic condition; Blood; Reversed phase chromatography; Analysis method; Fluorescent labelling; Clinical biology; Blood serum
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(98)00527-5

A fluorescent labelling reagent, 4-(5,6-dimethoxy-2-phthalimidinyl)phenylsulfonyl semipiperazide, was designed for the determination of carboxylic acids by precolumn HPLC. The reagent was reacted with carboxylic acids at 100°C for 15 min in the presence of an activating reagent (a mixture of triphenylphosphine, 2,2′-dipyridyldisulfide and pyridine) and produced highly fluorescent derivatives, which were separated on a reversed-phase column by fluorescence measurement at 317 nm (excitation) and 380 nm (emission). The detection limits were 4–12 fmol/injection. The reagent was used for HPLC assays of long chain fatty acids in human serum by isocratic elution.