Reversible shape change in platelets is regulated by phosphatidylinositol metabolism

Reversible platelet activation was studied after mechanical activation by low speed centrifugation (600 xg). Immediately following centrifugation platelets exhibited no shape change response to low doses of thrombin, collagen and ADP. After incubation at 37°C a time-dependent recovery of the shape c...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 141; no. 2; pp. 673 - 681
Main Authors Dimitrov, D.P., Bühler, F.R.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 15.12.1986
Elsevier
Subjects
Applied sciences
Blood Platelets - metabolism
Blood Platelets - ultrastructure
Centrifugation
Exact sciences and technology
Humans
In Vitro Techniques
Membrane Lipids - blood
Other techniques and industries
Phosphatidic Acids - blood
Phosphatidylcholines - blood
Phosphatidylinositols - blood
Platelet Aggregation
Thrombin - pharmacology
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Summary:Reversible platelet activation was studied after mechanical activation by low speed centrifugation (600 xg). Immediately following centrifugation platelets exhibited no shape change response to low doses of thrombin, collagen and ADP. After incubation at 37°C a time-dependent recovery of the shape change response was observed. This was accompanied by a 50% decrease of 32P-incorporation into phosphatidic acid (PA) phosphotidylinositolmonophosphate (PIP), but not PIP 2, relative to levels observed immediately after centrifugation. After 60 minutes platelet relaxation was complete: [ 32]PA and [ 32]PIP reached lowest levels and the shape change response to low doses of thrombin was completely restored.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(86)80225-X