New steroids from the pericarps of Datura metel L

• Published in: Natural product research Vol. 38; no. 9; pp. 1553 - 1561
• Main Authors: Liu, Yan, Liu, Yuan, Zou, Hai-dan, Zhou, Yong-qiang, Pan, Juan, Guan, Wei, Algradi, Adnan Mohammed, Yang, Bing-you, Kuang, Hai-xue
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.05.2024; Taylor & Francis Ltd
• Subjects: Analogs; Animals; anti-inflammatory activity; Anti-inflammatory agents; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Datura metel; Datura metel - chemistry; Datura metel L. pericarps; Inflammation; Magnetic resonance spectroscopy; Mice; NMR; NMR spectroscopy; Nuclear magnetic resonance; Plant Extracts - chemistry; RAW 264.7 Cells; Spectroscopy; Steroid hormones; Steroids; Steroids - pharmacology; Structural analysis; Datura metel L. pericarps; RAW 264.7 cells; Steroids; anti-inflammatory activity
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2022.2158461

Three new steroids (1-3) and 13 reported analogs (4-16) were extracted from Datura metel L. pericarps. Structure analysis of these extracted compounds was performed by 1 D-NMR and 2 D-NMR spectroscopy, and their spectra were compared with those of similar compounds previously described in the literature. The extracted steroids (1-3) and known compounds (4-16) were evaluated for anti-inflammatory activity against LPS-activated RAW 264.7 cells. Compounds 5, 7, 9, 12 and 15 showed potential anti-inflammatory activity with IC 50 less than 35 μM, while compounds 3 and 11 showed weak anti-inflammatory activity with IC 50 less than 100 μM.