Multidimensional counter-current chromatographic system and its application

• Published in: Journal of Chromatography A Vol. 803; no. 1; pp. 298 - 301
• Main Authors: Yang, Fuquan, Quan, Jiang, Zhang, Tian You, Ito, Yoichiro
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 17.04.1998; Elsevier
• Subjects: Aglycones; Analytical chemistry; Antiviral Agents - analysis; Antiviral Agents - chemistry; Biological and medical sciences; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Liquid - instrumentation; Chromatography, Liquid - methods; Cycadopsida - chemistry; Exact sciences and technology; Flavone aglycones; Flavonoids - analysis; Flavonoids - chemistry; Flavonols; General pharmacology; Isorhamnetin; Kaempferol; Kaempferols; Medical sciences; Other chromatographic methods; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Quercetin; Quercetin - analogs & derivatives; Quercetin - analysis; Quercetin - chemistry; Ginkgo biloba; Multidimensional separation; Isorhamnetin; Hippophae rhamnoides; Counter-current chromatography; Kaempferol; Aglycones; Quercetin; Flavone aglycones; Multidimensional chromatography; Countercurrent chromatography; Purification; Pharmacognosy; Preparative chromatography; Aglycone; Flavonoid; Medicinal plant; Polyphenol; Elaeagnaceae; Dicotyledones; Angiospermae; Gymnospermae; Plant origin; Ginkgoales; Spermatophyta
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(97)01273-9

A multidimensional counter-current chromatographic system was set up for the first time with two sets of high-speed counter-current chromatography instruments. This system was successfully applied to the preparative separation of isorhamnetin, kaempferol and quercetin from crude flavone aglycones of Ginkgo biloba L. and Hippophae rhamnoides L. with a two-phase solvent system composed of chloroform–methanol–water (4:3:2, v/v/v).