Identification by subtractive hybridization of a spectrum of novel and unexpected genes associated with in vitro differentiation of human cytotrophoblast cells

• Published in: Placenta (Eastbourne) Vol. 17; no. 7; pp. 431 - 441
• Main Authors: Morrish, D.W., Linetsky, E., Bhardwaj, D., Li, H., Dakour, J., Marsha, R.G., Paterson, M.C., Godbout, R.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.09.1996; Elsevier
• Subjects: 3-Hydroxysteroid Dehydrogenases - genetics; Biological and medical sciences; Cell Differentiation - genetics; Embryology: invertebrates and vertebrates. Teratology; Epidermal Growth Factor - pharmacology; Female; Fetal membranes; Fibronectins - genetics; Fundamental and applied biological sciences. Psychology; General aspects. Development. Fetal membranes; Glycoprotein Hormones, alpha Subunit - genetics; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Plasminogen Activator Inhibitor 1 - genetics; Pregnancy; Pregnancy-Specific beta 1-Glycoproteins - genetics; RNA, Messenger - metabolism; Superoxide Dismutase - genetics; Trophoblasts - cytology; Human; Trophoblaste; Regulation(control); Epidermal growth factor; Placenta; Gene; Fetal membrane; Female; Differentiation; In vitro
• ISSN: 0143-4004; 1532-3102
• DOI: 10.1016/S0143-4004(96)90025-9

We have previously demonstrated that epidermal growth factor (EGF), colony stimulating factor-1 (CSF-1), and granulocytemonocyte colony stimulating factor (GMCSF) stimulate, while transforming growth factor β1 (TGFβ1) inhibits, cytotrophoblast differentiation. To identify genes mediating EGF-induced differentiation,we constructed a subtracted cDNA library between undifferentiated cytotrophoblast and differentiating cytotrophoblast. We identified six novel genes and four known syncytial products α-human chorionic gonadotrophin (αhCG) pregnancy-specific β1-glycoprotein, 3β-hydroxysteroid dehydrogenase, and plasminogen activator inhibitor type 1 whose mRNAs increased during differentiation. Ten other genes were identified whose mRNAs increased during differentiation. Five of these (keratin 19, calcreticulin, heat shock protein 27, serum and glucocorticoid-regulated kinase and adrenomedullin) were not previously reported to be expressed in placenta. Five other genes known to be expressed in placenta were identified: keratin S, fibronectin, mitochondrial ATP synthase, H19, and cytosolic copper-zinc superoxide dismutase (SOD-1). Several of these genes may have regulatory functions in trophoblast differentiation.