Distribution of ciprofloxacin-resistance genes among ST131 and non-ST131 clones of Escherichia coli isolates with ESBL phenotypes isolated from women with urinary tract infection
sequence type 131 (ST131) is associated with extended-spectrum beta-lactamase (ESBL) production and fluoroquinolone resistance. This study aimed to investigate the prevalence of ST131, ESBL, and plasmid-mediated quinolone resistance (PMQR) genes in the ciprofloxacin-resistant (CIP ) and ESBL produce...
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Published in | Iranian journal of microbiology Vol. 13; no. 3; pp. 294 - 302 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Iran
Tehran University of Medical Sciences
01.06.2021
|
Subjects | |
Online Access | Get full text |
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Summary: | sequence type 131 (ST131) is associated with extended-spectrum beta-lactamase (ESBL) production and fluoroquinolone resistance. This study aimed to investigate the prevalence of ST131, ESBL, and plasmid-mediated quinolone resistance (PMQR) genes in the ciprofloxacin-resistant (CIP
) and ESBL producers from women with UTI.
The CIP-resistant ESBL producing (CIP
/ESBL
)
isolates were screened for ST131-by specific PCR of
and
. The ESBL and PMQR genes were screened by single PCR. The ST131 and non-ST131 isolates were selected to determine the mutations of
and
using PCR and sequencing, and also their genetic background by the Pasteur-MLST scheme.
Overall, 55% (33/60) CIP
/ESBL
isolates were identified as ST131 (94% O25b-ST131). Resistance rate to ampicillin-sulbactam (70%), aztreonam (97%) and gentamicin (61%), the prevalence of
(66%),
(82%), the profile of
(30%), and the double mutation in the
was significantly higher in ST131 than non- ST131 isolates. The coexistence of PMQR and ESBL genes was found in more than 50% of ST131 and non-ST131 isolates. ST131 isolates differentiated into PST43 and PST506.
Management of women with UTI caused by the CIP
/ESBL
isolates (ST131) co-harbored PMQR, ESBL, and chromosomal mutations, is important for their effective therapy. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2008-3289 2008-4447 |
DOI: | 10.18502/ijm.v13i3.6389 |