Isolation and sequencing of a new glucoamylase gene from an Aspergillus niger aggregate strain (DSM 823) molecularly classified as Aspergillus tubingensis

Based on morphological characteristics the taxa included in the Aspergillus aggregate can hardly be differentiated. For that reason the phylogeny of this genus was revised several times as different criteria, from morphological to later molecular, were used. We found, comparing nucleotide sequences...

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Bibliographic Details
Published inAntonie van Leeuwenhoek Vol. 88; no. 3-4; pp. 267 - 275
Main Authors Manger-Jacob, Friederike, Müller, Tobias, Janssen, Martina, Höfer, Milan, Hölker, Udo
Format Journal Article
LanguageEnglish
Published Netherlands Springer Nature B.V 01.10.2005
Subjects
Amino Acid Sequence
Amino acids
Aspergillus kawachii
Aspergillus niger
Aspergillus niger - classification
Aspergillus niger - enzymology
Aspergillus niger - genetics
Aspergillus tubingensis
Bacteria
Bacteriology
Base Sequence
Cloning, Molecular
DNA, Fungal - chemistry
DNA, Fungal - genetics
DNA, Ribosomal Spacer - chemistry
DNA, Ribosomal Spacer - genetics
Genes, Fungal
Glucan 1,4- alpha -glucosidase
Glucan 1,4-alpha-Glucosidase - chemistry
Glucan 1,4-alpha-Glucosidase - genetics
Microbiology
Molecular Sequence Data
Molecular Weight
Nucleotide sequence
Open Reading Frames
Phylogeny
Primers
Sequence Analysis, DNA
Sequence Homology, Amino Acid
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Summary:Based on morphological characteristics the taxa included in the Aspergillus aggregate can hardly be differentiated. For that reason the phylogeny of this genus was revised several times as different criteria, from morphological to later molecular, were used. We found, comparing nucleotide sequences of the ITS-region, that the strain Aspergillus niger (DSM 823) which is claimed to be identical to the strains ATCC 10577, IMI 027809, NCTC 7193 and NRRL 2322 can be molecularly classified as Aspergillus tubingensis, exhibiting 100% identity with the A. tubingensis CBS strains 643.92 and 127.49. We amplified, cloned and sequenced a new glucoamylase gene (glaA) from this strain of A. tubingensis (A. niger DSM 823) using primers derived from A. niger glucoamylase G1. The amplified cDNA fragment of 2013 bp contained an open reading frame encoding 648 amino acid residues. The calculated molecular mass of the glucoamylase, deduced from the amino acid sequence, was 68 kDa. The nucleotide sequence of glaA showed 99% similarity with glucoamylases from Aspergillus kawachii and Aspergillus shirousami, whereas the similarity with the glucoamylase G1 from A. niger was 92%
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ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-005-3989-5