Qualitative detection of haptoglobin mRNA in bovine and human blood leukocytes and bovine milk somatic cells

• Published in: Veterinární medicína Vol. 50; no. 12; pp. 515 - 520
• Main Authors: Thielen, M.A.(University of Bonn (Germany). Inst. of Animal Science), Mielenz, M.(University of Bonn (Germany). Inst. of Animal Science), Hiss, S.(University of Bonn (Germany). Inst. of Animal Science), Sauerwein, H.(University of Bonn (Germany). Inst. of Animal Science) E-mail:sauerwein@uni-bonn.de
• Format: Journal Article
• Language: English
• Published: Prague Czech Academy of Agricultural Sciences (CAAS) 01.12.2005; Czech Academy of Agricultural Sciences
• Subjects: Alveoli; ANIMAL PHYSIOLOGY; ARN MENSAJERO; ARN MESSAGER; blood; Blood cells; cattle; CELLS; CELLULE; CELULAS; COW MILK; Cow's milk; DAIRY COWS; DEFENCE MECHANISMS; FISIOLOGIA ANIMAL; Gene expression; GENERO HUMANO; GENRE HUMAIN; HAPTOGLOBIN; haptoglobin mrna; human; LAIT DE VACHE; LECHE DE VACA; LEUCOCITOS; LEUCOCYTE; LEUKOCYTES; Mammary gland; Mammary glands; MANKIND; MECANISME DE DEFENSE; MECANISMOS DE DEFENSA; MESSENGER RNA; Milk; PCR; PHYSIOLOGIE ANIMALE; Polymerase chain reaction; PROTEINAS; PROTEINE; PROTEINS; Somatic cells; Transcription; VACAS LECHERAS; VACHE LAITIERE
• ISSN: 0375-8427; 1805-9392
• DOI: 10.17221/5658-VETMED

The aim of this study was to investigate whether bovine leukocytes might contribute to the Hp mRNA transcripts found in the mammary gland after their migration into this organ. For this purpose RT-PCR was carried out with total RNA extracted from leukocytes in blood and from somatic cells in milk of three dairy cows. In comparison, human leukocytes of four donors were examined. Both bovine blood and milk somatic cells showed distinct signals for Hp mRNA in all samples in contrast to human leukocytes where Hp mRNA was detected in only two out of four donors after reamplification. In conclusion, the presence of Hp mRNA in blood derived leukocytes indicates that these cells contribute to the Hp mRNA transcripts found in the bovine mammary gland. It remains to be clarified whether other cells within the mammary gland are also capable of Hp mRNA synthesis.