Cell Synchronization by Double Thymidine Block

Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. Here, we...

Full description

Saved in:
Bibliographic Details
Published inBio-protocol Vol. 8; no. 17
Main Authors Chen, Guo, Deng, Xingming
Format Journal Article
LanguageEnglish
Published United States Bio-Protocol 05.09.2018
Bio-protocol LLC
Subjects
Biology
Methods
DNA synthesis
DNA replication
Thymidine
Cell synchronization
Cell cycle
Online AccessGet full text

Cover

More Information
Summary:Cell synchronization is widely used in studying mechanisms involves in regulation of cell cycle progression. Through synchronization, cells at distinct cell cycle stage could be obtained. Thymidine is a DNA synthesis inhibitor that can arrest cell at G1/S boundary, prior to DNA replication. Here, we present the protocol to synchronize cells at G1/S boundary by using double thymidine block. After release into normal medium, cell population at distinct cell cycle phase could be collected at different time points.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.2994