An integrated microfluidic system with field-effect-transistor sensor arrays for detecting multiple cardiovascular biomarkers from clinical samples

• Published in: Biosensors & bioelectronics Vol. 129; pp. 155 - 163
• Main Authors: Sinha, Anirban, Tai, Tse-Yu, Li, Kuang-Hsien, Gopinathan, Priya, Chung, Yi-Da, Sarangadharan, Indu, Ma, Hsi-Pin, Huang, Po-Chiun, Shiesh, Shu-Chu, Wang, Yu-Lin, Lee, Gwo-Bin
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.03.2019
• Subjects: Aptamers; Biomarkers; biosensors; C-reactive protein; Cardiovascular diseases; fibrinogen; Field-effect transistors; Microfluidics; natriuretic peptides; oligonucleotides; point-of-care systems; portable equipment; prototypes; risk; transistors; troponin I; CRP; cTnI; HEMT; TE buffer; EDL; AlGaN/GaN; NT-proBNP; SPR; ssDNA; SELEX; LOD; PBS; EMVs; EDTA; LCD; FET; PMMA; Field-effect transistors; Aptamers; Biomarkers; Cardiovascular diseases; CVDs; TCEP; Microfluidics; ELISA; PDMS
• ISSN: 0956-5663; 1873-4235; 1873-4235
• DOI: 10.1016/j.bios.2019.01.001

Certain blood-borne biomarkers offer a potent methodology for understanding the risk of cardiovascular diseases (CVDs) with clinicians generally advocating the use of multiple biomarkers for proper risk assessment of CVDs. Herein four such CVDs biomarkers- C-reactive protein (CRP), N-terminal pro b-type natriuretic peptide (NT-proBNP), cardiac troponin I (cTnI), and fibrinogen- were rapidly (5 min) analyzed from clinical samples (~ 4 µL) on an integrated microfluidic platform equipped with 1) immobilized highly specific aptamer probes and 2) field-effect transistor (FET)-based sensor arrays. The calibration curve from the FET sensor arrays showed good agreement in the physiological concentration ranges for CRP (0.1–50 mg/L), NT-proBNP (50–10,000 pg/mL), cTnI (1–10,000 pg/mL), and fibrinogen (0.1–5 mg/mL). The developed prototype of this fully automated portable device requires minimal reagent and sample inputs and consequently shows great promise for next-generation point-of-care devices assaying multiple CVDs biomarkers in clinical samples. •An integrated microfluidic platform equipped with 1) immobilized aptamer probes and 2) field-effect transistor (FET)-based sensor arrays has been presented in this study.•Four biomarkers for cardiovascular diseases were rapidly (5 min) analyzed from clinical samples (~ 4 µL).•The developed prototype of this fully automated portable device requires minimal reagent and sample inputs and consequently shows great promise for next-generation point-of-care devices assaying multiple CVDs biomarkers in patient serum.