E-cadherin and its messenger ribonucleic acid in periimplantation phase human endometrium in normal and clomiphene-treated cycles

• Published in: American journal of obstetrics and gynecology Vol. 178; no. 5; pp. 996 - 1001
• Main Authors: Dawood, M.Yusoff, Lau, Manhot, Khan-Dawood, Firyal S.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Philadelphia, PA Mosby, Inc 01.05.1998; Elsevier
• Subjects: Adult; Biological and medical sciences; Biopsy; Blotting, Western; Cadherins - analysis; Cadherins - genetics; Cadherins - metabolism; Clomiphene - pharmacology; clomiphene citrate; E-cadherin; Embryo Implantation; Endometrium; Endometrium - drug effects; Endometrium - metabolism; Female; Genital system. Reproduction; Humans; Immunohistochemistry; Medical sciences; messenger ribonucleic acid; periimplantation phase; Pharmacology. Drug treatments; Polymerase Chain Reaction; Pregnancy; Progesterone - blood; RNA, Messenger - analysis; RNA, Messenger - metabolism; RNA-Directed DNA Polymerase; periimplantation phase; clomiphene citrate; messenger ribonucleic acid; E-cadherin; Endometrium; Surgical biopsy; Human; Immunohistochemistry; Non steroidal estrogen; Embryo; Ovulation inducer; Cell adhesion molecule; Clomifene; Gene expression; Pathology; Chemotherapy; Messenger RNA; Treatment; Ovum implantation; Surgery; Immunoblotting assay; Female; Influence; Molecular biology; E Cadherin
• ISSN: 0002-9378; 1097-6868
• DOI: 10.1016/S0002-9378(98)70538-1

OBJECTIVE: The purpose of this investigation was to determine whether treatment with clomiphene citrate, which is estrogenic and antiestrogenic, affects the expression of the cell adhesion molecule E-cadherin in human periimplantation phase endometrium. STUDY DESIGN: Five healthy women were studied for two cycles each, a control and a treated (clomiphene 50 mg daily, days 5 through 9) cycle. A biopsy specimen of endometrial tissue was studied (8 to 10 days post luteinizing hormone surge) for immunohistochemical localization, Western analysis of E-cadherin with use of a highly specific monoclonal antibody to human E-cadherin, and determination of messenger ribonucleic acid for E-cadherin by reverse transcription–polymerase chain reaction by use of oligonucleotide primers specific to E-cadherin and amplifying a 432 bp fragment. RESULTS: Luteal phase plasma progesterone levels were significantly higher in clomiphene cycles. E-cadherin was immunocytochemically present in endometrium of control and treated cycles with no apparent difference in staining intensity. Western blots revealed the presence of E-cadherin. It was relatively more abundant in clomiphene-treated than control cycles but not significantly different. The message for E-cadherin gene is expressed in endometrium of control ( n = 5) and clomiphene cycles ( n = 4). CONCLUSIONS: E-cadherin and its gene transcripts are expressed in periimplantation phase endometrium and are not significantly affected by clomiphene treatment. (Am J Obstet Gynecol 1998;178:996-1001.)