MicroRNA‑19a‑3p inhibits the cellular proliferation and invasion of non‑small cell lung cancer by downregulating UBAP2L

• Published in: Experimental and therapeutic medicine Vol. 20; no. 3; pp. 2252 - 2261
• Main Authors: Pan, Yuejiang, Jin, Ke, Xie, Xuan, Wang, Kexi, Zhang, Huizhong
• Format: Journal Article
• Language: English
• Published: Athens Spandidos Publications 01.09.2020; Spandidos Publications UK Ltd; D.A. Spandidos
• Subjects: Apoptosis; Breast cancer; Cancer metastasis; Cancer therapies; Cell adhesion & migration; Cell growth; Chemotherapy; Comparative analysis; Development and progression; Gene expression; Kinases; Liver cancer; Lung cancer; Lymphoma; MicroRNA; MicroRNAs; Non-small cell lung cancer; Phase transitions; Prostate cancer; Radiation therapy; Small cell lung cancer; Surgery; Wound healing; China; United States > US
• ISSN: 1792-0981; 1792-1015
• DOI: 10.3892/etm.2020.8926

MicroRNAs (miRNAs) are increasingly recognized as important regulators of non-small cell lung cancer (NSCLC) progression by directly regulating their target genes. The aim of the present study was to assess the biological role of miR-19a-3p in NSCLC. It was revealed that miR-19a-3p expression was significantly downregulated in human NSCLC tissues and cell lines compared with normal tissues and lung epithelial cells. In addition, a lower miR-19a-3p expression was significantly associated with Tumor Node Metastasis stage and lymph node metastasis. Furthermore, the upregulation of miR-19a-3p in NSCLC cell lines significantly inhibited cell proliferation, migration and invasion, as determined using an MTT, colony formation, wound healing and transwell Matrigel invasion assays, respectively. A luciferase reporter assay and western blotting determined that ubiquitin associated protein 2 like (UBAP2L) was a direct target of miR-19a-3p and could be inhibited through the upregulation of miR-19a-3p in NSCLC. In addition, UBAP2L silencing induced similar effects to those observed following miR-19a-3p overexpression. The overexpression of UBAP2L partially reversed the effects of miR-19a-3p on NSCLC cell lines. Collectively, these data indicated that miR-19a-3p may serve as a tumor suppressor partly through the regulation of UBAP2L expression in NSCLC and that the targeting of miR-19a-3p may be a novel method for NSCLC treatment.