Supercritical fluid extraction of Rumex Acetosa L. roots: Yield, composition, kinetics, bioactive evaluation and comparison with conventional techniques

• Published in: The Journal of supercritical fluids Vol. 122; pp. 1 - 9
• Main Authors: Santos, Ênio R.M., Oliveira, Humberto N.M., Oliveira, Eduardo J., Azevedo, Saulo H.G., Jesus, Anderson A., Medeiros, Aldo M., Dariva, Claudio, Sousa, Elisa M.B.D.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.04.2017
• Subjects: Antioxidant activity; Emodin; Phenolic compounds; Rumex acetosa L; Supercritical fluid extraction; Trans-resveratrol; Emodin; Trans-resveratrol; Phenolic compounds; Supercritical fluid extraction; Antioxidant activity; Rumex acetosa L
• ISSN: 0896-8446; 1872-8162
• DOI: 10.1016/j.supflu.2016.11.019

[Display omitted] •SFE of Rumex Acetosa roots using CO2 and ethanol as cossolvent was investigated.•Effects of extraction process variables were evaluated on yields and compositions.•The highest yield was obtained at 15MPa, 40°C with a 35% of a EtOH/CO2 solvent mixture.•The use of ethanol as co-solvent increased the extraction yield of phenolic compounds.•SFE process favored the selective extraction of Emodin and Resveratrol compounds on the extracts. In this study, Rumex Acetosa extracts were obtained from extraction techniques with supercritical fluid (SFE), Soxhlet and low pressure solvent extraction (LPSE). In the SFE, it was evaluated the effect of the process variables (temperature, solvent flow rate, pressure and cosolvent concentration) on the extraction kinetics, yield and extract solubility. In the Soxhlet extraction, it was evaluated the usage of ethanol, acetonitrile, isopropanol and tert-butyl alcohol, while in LPSE extraction, it was used ethanol as solvent. The phenolics total content (TPC), antioxidant capacity (IC50) and extract composition were determined. The yield obtained ranged from 0.2 to 8.3%. The SFE extraction has demonstrated great efficiency in the extraction of phenolic compounds (anthraquinone and stilbene). The trans-resveratrol concentration in the extracts ranged between 1.03 and 3.52mg/g, depending on the technique used, the values of IC50 and TPC ranges from ​ 10.67 to 163.43μg/mL and 27.63 to 157.04 mgEAG/g, respectively.