A radiation hybrid map of the X-chromosome of the dog (Canis familiaris)

• Published in: Cytogenetic and genome research Vol. 98; no. 1; pp. 86 - 92
• Main Authors: Everts, R.E., van Wolferen, M.E., Versteeg, S.A., Zijlstra, C., Engelen, J.J.M., Bosma, A.A., Rothuizen, J., van Oost, B.A.
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.01.2002; S. Karger AG
• Subjects: Animal Cytogenetics and Comparative Mapping; Animals; Base Sequence; Biological and medical sciences; Chromosome Mapping; DNA Primers; Dogs - genetics; Fundamental and applied biological sciences. Psychology; Genes. Genome; Genetic Markers; Humans; In Situ Hybridization, Fluorescence; Microsatellite Repeats - genetics; Molecular and cellular biology; Molecular genetics; Polymerase Chain Reaction; Radiation Hybrid Mapping - methods; Species Specificity; X Chromosome; Genetic mapping; Fissipedia; Carnivora; Hybrid; Canis familiaris; Vertebrata; Mammalia; Gene; Microsatellite DNA; X-Chromosome; Genome; Dog; Sequence tagged site
• ISSN: 1424-8581; 1424-859X
• DOI: 10.1159/000068547

The dog serves as an animal model for several human diseases including X-chromosome diseases. Although the canine X-chromosome is one of the largest chromosomes in the dog, only a few markers have been mapped to it to date. Using a commercially available canine whole genome radiation hybrid (RH) panel we have localized 14 microsatellite markers, 18 genes and 13 STSs on the canine X-chromosome, extending the total number of mapped markers to 45 covering an estimated 830 cR. Out of these 45 markers, seven distinct groups of markers could be established with an average spacing of 18.8 cR 3000 and ten markers remained unlinked. Using FISH analysis, six markers could be mapped physically to the p- or q-arm of the X-chromosome. Combined with the FISH mapping, three RH groups could be assigned to the p-arm and two RH groups to the q-arm. Comparison with the human X-chromosome map revealed conserved synteny up to 234 cR (TIMP1–ALAS2–AR–IL2RG–XIST). We show here that the similarity of the canine and human X-chromosomes is the largest for any mammalian species beyond the primates.