Low temperature inhibits root growth by reducing auxin accumulation via ARR1/12

• Published in: Plant and cell physiology Vol. 56; no. 4; pp. 727 - 736
• Main Authors: Zhu, Jiang, Zhang, Kun-Xiao, Wang, Wen-Shu, Gong, Wen, Liu, Wen-Cheng, Chen, Hong-Guo, Xu, Heng-Hao, Lu, Ying-Tang
• Format: Journal Article
• Language: English
• Published: Japan 01.04.2015
• Subjects: Arabidopsis - cytology; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Cell Count; Cell Division; Cold Temperature; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Gene Expression Regulation, Plant; Indoleacetic Acids - metabolism; Meristem - cytology; Meristem - growth & development; Meristem - metabolism; Plant Roots - cytology; Plant Roots - growth & development; Plant Roots - metabolism; Stress, Physiological; Transcription Factors - genetics; Transcription Factors - metabolism; Auxin; Low temperature; Root meristem
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/pcp/pcu217

Plants exhibit reduced root growth when exposed to low temperature; however, how low temperature modulates root growth remains to be understood. Our study demonstrated that low temperature reduces both meristem size and cell number, repressing the division potential of meristematic cells by reducing auxin accumulation, possibly through the repressed expression of PIN1/3/7 and auxin biosynthesis-related genes, although the experiments with exogenous auxin application also suggest the involvement of other factor(s). In addition, we verified that ARABIDOPSIS RESPONSE REGULATOR 1 (ARR1) and ARR12 are involved in low temperature-mediated inhibition of root growth by showing that the roots of arr1-3 arr12-1 seedlings were less sensitive than wild-type roots to low temperature, in terms of changes in root length and meristem cell number. Furthermore, low temperature reduced the levels of PIN1/3 transcripts and the auxin level to a lesser extent in arr1-3 arr12-1 roots than in wild-type roots, suggesting that cytokinin signaling is involved in the low-temperature-mediated reduction of auxin accumulation. Taken together, our data suggest that low temperature inhibits root growth by reducing auxin accumulation via ARR1/12.