Smartphone-based colorimetric detection of cardiac troponin T via label-free aptasensing

We report an aptasensing platform for the detection of cardiac troponin T (cTnT) in the immediate and early phases of acute myocardial infarction (AMI). High-flow filter paper was used to fabricate a microfluidic paper-based analytical device (μ-PAD), which was further modified with gold-decorated p...

Full description

Saved in:
Bibliographic Details
Published inBiosensors & bioelectronics Vol. 222; p. 114938
Main Authors Ulloa-Gomez, Ana M., Agredo, Alejandra, Lucas, Alec, Somvanshi, Sandeep B., Stanciu, Lia
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 15.02.2023
Subjects
Aptasensor
aptasensors
Biomarkers
Biosensing Techniques
blood serum
Cardiac troponin T
Colorimetric
Colorimetry
cost effectiveness
Humans
image analysis
interleukin-6
myocardial infarction
Myocardial Infarction - diagnosis
oligonucleotides
Paper-based
polystyrenes
Smartphone
Smartphone-based
Troponin T
tumor necrosis factor-alpha
Paper-based
Aptasensor
Cardiac troponin T
Smartphone-based
Colorimetric
Online AccessGet full text

Cover

More Information
Summary:We report an aptasensing platform for the detection of cardiac troponin T (cTnT) in the immediate and early phases of acute myocardial infarction (AMI). High-flow filter paper was used to fabricate a microfluidic paper-based analytical device (μ-PAD), which was further modified with gold-decorated polystyrene microparticles functionalized with a highly specific cTnT aptamer. Herein, cTnT detection is presented in two linear ranges (0.01-0.8 μg/ml and 6.25-50 μg/ml) with an LoD of 3.9X10−4 μg/ml, which is in agreement with reference values determined by the American Heart Association. The proposed platform showed remarkable selectivity against AMI-associated cardiac biomarkers such as TNF-alpha, interleukin-6, cardiac troponin I, and reactive protein-C. This aptasensor is a label-free assay that relies only on smartphone-based image analysis and takes less processing time in comparison with traditional methods like ELISA. Furthermore, it exhibits outstanding stability over 23 days when devices are stored at 4 °C. The reported platform is a stable and cost-effective method for the on-site and user-friendly detection of cTnT in normal saline buffer and diluted human serum.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0956-5663
1873-4235
1873-4235
DOI:10.1016/j.bios.2022.114938