Simultaneous measurement of tyrosine, tryptophan and related monoamines for determination of neurotransmitter turnover in discrete rat brain regions by liquid chromatography with electrochemical detection

Concomitant measurement of monoamine neurotransmitter turnover in discrete rat brain areas with the use of radiolabeled amino acid precursors permits simultaneous evaluation of interacting transmitter systems. [ 3H] Tyrosine and [ 3H] tryptophan were administered via indwelling catheters to unrestra...

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Bibliographic Details
Published inJournal of chromatography Vol. 305; no. 1; pp. 27 - 42
Main Authors Lasley, Stephen M., Arthur Michaelson, I., Greenland, Robert D., McGinnis, Patricia M.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.01.1984
Subjects
Animals
Biogenic Amines - analysis
Brain Chemistry
Chromatography, Liquid - methods
Electrochemistry
Male
Neurotransmitter Agents - analysis
Rats
Serotonin - analysis
Tryptophan - analysis
Tyrosine - analysis
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Summary:Concomitant measurement of monoamine neurotransmitter turnover in discrete rat brain areas with the use of radiolabeled amino acid precursors permits simultaneous evaluation of interacting transmitter systems. [ 3H] Tyrosine and [ 3H] tryptophan were administered via indwelling catheters to unrestrained rats. Content and specific activity of norepinephrine, dopamine, 5-hydroxytryptamine, and the metabolites dihydroxyphenylacetic acid, homovanillic acid, and 5-hydroxyindoleacetic acid in addition to tyrosine and tryptophan were quantified by liquid chromatography with electrochemical detection and liquid scintillation counting. The method employs a simple extraction procedure without prior cleanup for chromatography. Neurotransmitter turnover rates that incorporated tyrosine- or tryptophanspecific activities were found to be two to four times greater than those that did not include them.
ISSN:0378-4347
DOI:10.1016/S0378-4347(00)83311-3