Prevotella nanceiensis sp. nov., isolated from human clinical samples

• Published in: International journal of systematic and evolutionary microbiology Vol. 57; no. 10; pp. 2216 - 2220
• Main Authors: Alauzet, C, Mory, F, Carlier, J.-P, Marchandin, H, Jumas-Bilak, E, Lozniewski, A
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.10.2007; Society for General Microbiology; Microbiology Society
• Subjects: Anaerobiosis - physiology; Bacterial Proteins - genetics; Bacterial Typing Techniques; Bacteriology; Bacteroidaceae Infections - microbiology; Base Composition; Biological and medical sciences; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; DNA-Directed RNA Polymerases - genetics; Electrophoresis, Gel, Pulsed-Field; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Humans; Life Sciences; Microbiology; Microbiology and Parasitology; Miscellaneous; Molecular Sequence Data; Phylogeny; Pigments, Biological - biosynthesis; Prevotella - classification; Prevotella - genetics; Prevotella - isolation & purification; Prevotella - physiology; Prevotella nanceiensis; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Systematics; Human; Bacteria; New species; Prevotella; Taxonomy; Bacteroidaceae
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.65173-0

1 Université Nancy I, UMR CNRS 7565, UFR de Médecine, Laboratoire de Bactériologie, Vandoeuvre-les-Nancy, France 2 Centre Hospitalier et Universitaire de Nancy, Hôpital Central, Laboratoire de Bactériologie, Nancy, France 3 Institut Pasteur, Centre National de Référence des Bactéries Anaérobies et du Botulisme, Paris, France 4 Centre Hospitalier et Universitaire de Montpellier, Hôpital Arnaud de Villeneuve, Laboratoire de Bactériologie, Montpellier, France 5 Université Montpellier 1, UFR de Pharmacie, Laboratoire de Bactériologie-Virologie, EA 3755, Montpellier, France Correspondence A. Lozniewski a.lozniewski{at}chu-nancy.fr Three strains of anaerobic, non-pigmented, Gram-negative bacilli isolated from various human clinical samples were characterized in terms of phenotypic and genotypic tests, including sequence analysis of 16S rRNA and rpoB genes. The strains were most closely related to the type strains of Prevotella marshii and Prevotella shahii on the basis of both 16S rRNA (89.8 and 89.0 % identity, respectively) and rpoB gene sequences (83.1 and 82.8 % identity, respectively). Phylogenetic analysis showed that the isolates constituted a robust homogeneous group distinct from known species in the genus Prevotella . The rrn skeleton (as determined by PFGE) and the DNA G+C content, determined to be 39.4 mol% for strain LBN 293 T , distinguished the novel isolates from the type strains of P. marshii and P. shahii . The three strains were saccharolytic and produced acetic, lactic and succinic acids as major metabolic end products. Polyphasic investigations supported the proposal of a novel species, Prevotella nanceiensis sp. nov., with LBN 293 T (=AIP 261.03 T =CIP 108993 T =CCUG 54409 T ) as the type strain. Abbreviations: ML, maximum likelihood The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA and rpoB gene sequences determined in this study are respectively AY957555 and EF405531 for P. nanceiensis LBN 293 T , EF405529 and EF405532 for P. nanceiensis LBN 297 and EF405530 and EF405533 for P. nanceiensis LBN 298. Those for the rpoB gene sequences of P. marshii E9.34 T and P. shahii JCM 12083 T are respectively EF405534 and EF405535 . Transmission electron micrographs of cells of strain LBN 293 T and a comparison of its fatty acid profile with those of related type strains are available as supplementary material with the online version of this paper.