Synthesis of luminescent CePO4:Tb/Au composite for glucose detection

• Published in: Dyes and pigments Vol. 159; pp. 28 - 34
• Main Authors: Yan, Guanwen, Zhang, Yuan, Di, Weihua, Qin, Weiping
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.12.2018
• Subjects: CePO4:Tb/Au; Enzyme mimick; Glucose detection; Luminescence; Stability; CePO4:Tb/Au; Glucose detection; Stability; Enzyme mimick; Luminescence
• ISSN: 0143-7208; 1873-3743
• DOI: 10.1016/j.dyepig.2018.06.003

The glucose oxidase (GOx) is commonly used as targeting molecule for glucose detection since a specific enzymatic reaction can occur, namely, catalytic oxidation of glucose by GOx. However, the intrinsic denaturation of natural enzyme is a problem in the use that leads to the detection inaccuracy and unstability. Here, we designed and prepared the luminescent CePO4:Tb/Au composite for glucose sensing, in which small-sized Au nanoparticles possess GOx-mimicking activity for catalytic oxidation of glucose and the in situ generated H2O2 quenches the luminescence of CePO4:Tb, enabling the fluorescent detection of glucose. The quenching of luminescence in dilute solutions can be well described by the Stern-Volmer relationship in a wide analyte concentration range with the Ksv quenching constant of 13070 M−1. A detection limit of 5.25 μM and a good sensing selectivity were achieved. More importantly, the problem of unstability using natural enzyme in the glucose determination can be efficiently solved by using Au as catalyst instead of GOx due to long-term preserved catalytic activity of Au. Therefore, our developed fluorescent system for glucose detection is potential in the biochemical analysis. We designed and prepared the luminescent CePO4:Tb/Au for glucose sensing, in which small-sized Au nanoparticles possess GOx-mimicking activity for catalytic oxidation of glucose and the in situ generated H2O2 quenches the luminescence of CePO4:Tb. [Display omitted] •A new luminescent composite CePO4:Tb/Au for glucose detection was designed.•The detection limit of 5.25 μM for glucose was obtained.•High detection stability was achieved.•The problem of unstability using natural enzyme in the glucose determination can be solved.