Effects of 17 β estradiol on the metabolism of labelled arachidonic acid in uteri isolated from intact and ovariectomized rats. Influence of a restricted diet

• Published in: Prostaglandins, leukotrienes and essential fatty acids Vol. 56; no. 6; pp. 457 - 460
• Main Authors: Casalino-Matsuda, S.M., Linares, J.A., Goldraij, A.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.06.1997; Elsevier
• Subjects: 6-Ketoprostaglandin F1 alpha - biosynthesis; Animals; Arachidonic Acid - metabolism; Biological and medical sciences; Diet; Dinoprost - biosynthesis; Dinoprostone - biosynthesis; Estradiol - pharmacology; Female; Food Deprivation; Fundamental and applied biological sciences. Psychology; Hormone metabolism and regulation; Mammalian female genital system; Ovariectomy; Ovary - physiology; Rats; Rats, Wistar; Thromboxane B2 - biosynthesis; Uterus - drug effects; Uterus - metabolism; Vertebrates: reproduction; Prostaglandin; Rat; Arachidonic acid derivatives; Estrogen; Rodentia; 17β-Estradiol; Arachidonic acid; Metabolism; Ovarian hormone; Feeding; Vertebrata; Mammalia; Uterus; Eicosanoid; Female genital system; Low calorie diet
• ISSN: 0952-3278; 1532-2823
• DOI: 10.1016/S0952-3278(97)90599-8

The effects of restricted diet (50% of the normal intake during 25 days) on the metabolism of 14U arachidonic acid, were explored in uterine horn strips isolated from intact and ovariectomized rats, treated by 17 β-estradiol or controls. The metabolism of arachidonic acid into different eicosanoids, PGE 2, PGF 2α, 6-keto PGF 1α and TXB 2, showed that the restricted diet diminished PGE 2 and PGF 2α, in intact rats, significantly. In contrast, this kind of feeding did not produce any change in castrated rats. Tissue preparations from previously estrogenized intact and castrated normal-fed rats showed that the production of different metabolites decreased. A similar result was obtained in intact rats subjected to a restricted diet. Nevertheless, in castrated underfed rats, estrogens did not produce any effect on the various eicosanoids analysed. These results showed that in isolated uteri, the effects of 17 β-estradiol, on metabolite production from labelled arachidonic acid, are different from controls in ovariectomized diet-restricted rats.