Trisomy 21 and accelerated aging: DNA-repair parameters in peripheral lymphocytes of Down's syndrome patients

• Published in: Mechanisms of ageing and development Vol. 100; no. 1; pp. 85 - 101
• Main Authors: Raji, N.S, Rao, Kalluri Subba
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 12.01.1998; Elsevier Science
• Subjects: Adolescent; Adult; Aging - metabolism; Biological and medical sciences; Carbon-Oxygen Lyases - metabolism; Child; Child, Preschool; Chromatin. Chromosome; Deoxyribonuclease IV (Phage T4-Induced); DNA - biosynthesis; DNA Repair; DNA-(Apurinic or Apyrimidinic Site) Lyase; DNA-Directed DNA Polymerase - metabolism; DNA-polymerases; DNases; Down Syndrome - metabolism; Down's syndrome; Endodeoxyribonucleases - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Infant; Infant, Newborn; Leukocytes, Mononuclear - metabolism; Molecular and cellular biology; Molecular genetics; Multienzyme Complexes - metabolism; N-Glycosyl Hydrolases - metabolism; Premature aging; Unscheduled DNA synthesis; DNases; Premature aging; DNA-polymerases; Down's syndrome; Unscheduled DNA synthesis; DNA-repair; Chromosomal aberration; Human; Enzyme; Transferases; Ageing; Aneuploidy; DNA synthesis; Down syndrome; Congenital disease; Nucleotidyltransferases; Enzymatic activity; Repair; Lymphocyte; DNA-directed DNA polymerase
• ISSN: 0047-6374; 1872-6216
• DOI: 10.1016/S0047-6374(97)00121-8

Down's syndrome (DS) cases from 1–40 years of age and showing no other anomalies or deficiencies were categorized into three age groups: group 1, ⩽12 years; group 2, 13–25 years; and group 3, ⩾26 years. The DNA-repair markers like unscheduled DNA synthesis (UDS), activities of DNA polymerases, (Total, β and ε) and two endodeoxyribonucleases, (UV- and AP-DNases) were assessed in the peripheral lymphocytes of these subjects (under different conditions) along with age and sex matched normal healthy human subjects. The DS group showed lower DNA-repair efficiency and also an accelerated decline in DNA-repair capacity with age. These results indicate that deteriorated DNA-repair potential could be one of the probable reasons for premature aging seen in this chromosomal disorder.