New species emergence via recombination among isolates of the brazilian tomato infecting Begomovirus complex
Partial nucleotide sequences of five tomato infecting Begomovirus isolates were determined from DNA-A fragments, corresponding to the 5' region of the replication associated protein gene, the intergenic region and the 5' region of the coat protein gene. Isolate DFM shared 95% identity with...
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Published in | Pesquisa agropecuaria brasileira Vol. 41; no. 8; pp. 1329 - 1332 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Embrapa Informação TecnológicaPesquisa Agropecuária Brasileira
01.08.2006
Embrapa Secretaria de Pesquisa e Desenvolvimento; Pesquisa Agropecuária Brasileira |
Subjects | |
Online Access | Get full text |
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Summary: | Partial nucleotide sequences of five tomato infecting Begomovirus isolates were determined from DNA-A fragments, corresponding to the 5' region of the replication associated protein gene, the intergenic region and the 5' region of the coat protein gene. Isolate DFM shared 95% identity with Tomato mottle leaf curl virus (TMoLCV), isolates 34, PA-05, and Ta4 were 88% identical to Tomato yellow vein streak virus and isolate DF-BR3 shared 77% identity with TMoLCV. Recombination analysis indicated that isolate DF-BR3 was a chimaera, and it provided evidence that there is a complex and actively recombining population of tomato infecting begomoviruses in Brazil.
A seqüência de nucleotídeos parcial de cinco isolados de Begomovirus foi determinada do DNA-A, correspendente à região intergênica e à porção 5' do gene associado à replicação e da capa protéica. O isolado DFM apresentou identidade de 95% com Tomato mottle leaf curl virus (TMoLCV); os isolados 34, PA-05 e Ta4 foram 88% idênticos ao Tomato yellow vein streak virus (ToYVSV); e o isolado DF-BR3 mostrou 77% de identidade com TMoLCV. Análise de recombinação indicou que o isolado DF-BR3 seria uma quimera e evidencia que um complexo de espécies de begomovírus bipartidos está em formação no Brasil. |
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Bibliography: | 2006004305 H20 10.1590/S0100-204X2006000800018 http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000800018 |
ISSN: | 0100-204X 1678-3921 1678-3921 0100-204X |
DOI: | 10.1590/S0100-204X2006000800018 |