Identification of mycobacterial isolates by thin-layer and capillary gas-liquid chromatography under diagnostic routine conditions

The mycolic acid patterns of 75 strains of mycobacteria belonging to 46 different species were studied by thin-layer chromatography (TLC). Additionally, the mycolic acid pyrolytic cleavage products were determined by capillary gas-liquid chromatography (C-GLC). Eleven different patterns based on num...

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Bibliographic Details
Published inZentralblatt für Bakteriologie Vol. 278; no. 1; p. 34
Main Authors Yassin, A F, Binder, C, Schaal, K P
Format Journal Article
LanguageEnglish
Published Germany 01.02.1993
Subjects
Chromatography, Gas
Chromatography, Thin Layer
Mycobacterium - chemistry
Mycobacterium - classification
Mycolic Acids - analysis
Mycolic Acids - chemistry
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Summary:The mycolic acid patterns of 75 strains of mycobacteria belonging to 46 different species were studied by thin-layer chromatography (TLC). Additionally, the mycolic acid pyrolytic cleavage products were determined by capillary gas-liquid chromatography (C-GLC). Eleven different patterns based on number, type and Rf values of the detected lipid spots were identified. The mycolic acid methyl ester profiles of clinical isolates were compared with those of reference strains. In this way, it was possible to relate these clinical isolates to mycobacterial groups defined by their mycolic acid profiles. The 11 patterns and the methods used are described in detail.
ISSN:0934-8840
DOI:10.1016/S0934-8840(11)80277-X