Characteristics of six small heat shock protein genes from Bactrocera dorsalis: Diverse expression under conditions of thermal stress and normal growth

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol. 213; pp. 8 - 16
• Main Authors: Dou, Wei, Tian, Yi, Liu, Hong, Shi, Yan, Smagghe, Guy, Wang, Jin-Jun
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.11.2017
• Subjects: 20-Hydroxyecdysone; Animals; Bactrocera dorsalis; cold stress; complementary DNA; ecdysterone; eggs; fat body; Gene Expression Regulation - physiology; genes; heat; heat shock proteins; heat stress; Heat-Shock Proteins - biosynthesis; Heat-Shock Proteins - genetics; Heat-Shock Response - physiology; Insect Proteins - biosynthesis; Insect Proteins - genetics; larvae; Malpighian tubules; metamorphosis; molecular weight; open reading frames; pests; quantitative polymerase chain reaction; Small heat shock protein; Spatiotemporal expression; Tephritidae - genetics; Tephritidae - metabolism; Thermal stress; Time Factors; Thermal stress; Small heat shock protein; Spatiotemporal expression; 20-Hydroxyecdysone; Bactrocera dorsalis
• ISSN: 1096-4959; 1879-1107; 1879-1107
• DOI: 10.1016/j.cbpb.2017.07.005

To explore the functions of small heat shock proteins (sHsps) in relation to thermal stress and development in Bactrocera dorsalis (Hendel), one of the most economically important pest species attacking a wide range of fruits and vegetables, six full-length cDNAs of sHsp genes (BdHsp17.7, 18.4, 20.4, 20.6, 21.6 and 23.8) were cloned, and the expression patterns in different developmental stages and tissues, as well as in response to both thermal and 20-hydroxyecdysone (20E) exposures, were examined using real time quantitative PCR. The open reading frames (ORFs) of six sHsps are 453, 489, 537, 543, 567 and 630bp in length, encoding proteins with molecular weights of 17.7, 18.4, 20.4, 20.6, 21.6 and 23.8kDa, respectively. BdHsp18.4 and BdHsp20.4 maintained lower expression levels in both eggs and larvae, whereas remarkably up-regulated after the larval-pupal transformation, suggesting that these two sHsps may be involved in metamorphosis. Significant tissue specificity exists among sHsps: the highest expression of BdHsp20.6 and BdHsp23.8 in the Malpighian tubules and ovary, respectively, versus a peak in the fat body for others. BdHsp20.4 and BdHsp20.6 were significantly up-regulated by thermal stress. In contrast, BdHsp18.4 and BdHsp23.8 reacted only to heat stress. BdHsp17.7 and BdHsp21.6 were insensitive to both heat and cold stresses. The degree of sHsps response depends on intensity of 20E treatment, i.e., dose and time. These results strongly suggest functional differentiation within the sHsp subfamily in B. dorsalis. The physiological function of sHsp members under thermal stress and normal growth remains the subjects of further investigation.