High-performance liquid chromatographic separation of lens crystallins and their subunits

A high-performance liquid chromatography (HPLC) method for the separation of lens crystallins has been developed which utilizes molecular sieve HPLC. Also described is a rapid one-step separation of the lens crystallin subunits using a reversed-phase C4 column.

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Bibliographic Details
Published inJournal of chromatography Vol. 351; no. 1; p. 103
Main Authors Perry, R E, Abraham, E C
Format Journal Article
LanguageEnglish
Published Netherlands 1986
Subjects
Aging
Amino Acids - analysis
Animals
Chromatography, High Pressure Liquid
Crystallins - isolation & purification
Lens, Crystalline - analysis
Rats
Rats, Inbred Strains
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Summary:A high-performance liquid chromatography (HPLC) method for the separation of lens crystallins has been developed which utilizes molecular sieve HPLC. Also described is a rapid one-step separation of the lens crystallin subunits using a reversed-phase C4 column.
DOI:10.1016/S0021-9673(01)83476-2