Assessment of pollution of the Boca de Camichin Estuary in Nayarit (Mexico) and its influence on oxidative stress in Crassostrea corteziensis oysters

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Vol. 200; pp. 47 - 55
• Main Authors: Toledo-Ibarra, G.A., Díaz Resendiz, K.J.G., Ventura-Ramón, G.H., Romero-Bañuelos, C.A., Medina-Díaz, I.M., Rojas-García, A.E., Vega-López, A., Girón-Pérez, M.I.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2016
• Subjects: Acetylcholinesterase - metabolism; Animals; Boca de Camichín estuary; Catalase - metabolism; Contamination; Copper - metabolism; Copper - toxicity; Crassostrea - drug effects; Crassostrea - metabolism; Crassostrea corteziensis; Environmental Monitoring - methods; Estuaries; Geography; Gills - drug effects; Gills - metabolism; Glutathione Peroxidase - metabolism; Iron - metabolism; Lipid Peroxides - metabolism; Manganese - metabolism; Manganese - toxicity; Metals - metabolism; Metals - toxicity; Mexico; Mollusca; Naphthalenes - metabolism; Naphthalenes - toxicity; Oxidative Stress; Oyster; Polycyclic Aromatic Hydrocarbons - metabolism; Polycyclic Aromatic Hydrocarbons - toxicity; Reactive Oxygen Species - metabolism; Seasons; Superoxide Dismutase - metabolism; Water Pollutants, Chemical - metabolism; Water Pollutants, Chemical - toxicity; Water Pollution - analysis; ISE, Mexico, Nayarit; Oxidative stress; Oyster; Boca de Camichín estuary; Contamination
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/j.cbpa.2016.05.003

Boca de Camichin Estuary is one of the main producers of Crassostrea corteziensis oysters in Mexico, but the presence of pollutants can affect oyster production. Molluscs produce reactive oxygen species (ROS) in response to changes in the environment and pollution. These ROS induce oxidative damage in biomolecules. The main objective of this study was to evaluate pollution in the estuary and the subsequent oxidative stress in C. corteziensis oysters during the 2010 production cycle. For this aim, we performed monthly samplings in the oyster farms from January to May. We took water samples to quantify polycyclic aromatic hydrocarbon (PAH) and metal content; also, we evaluated oxidative damage (lipoperoxidation, lipidic hydroperoxides, protein oxidation) and enzyme activity (CAT, SOD, GPx, GST and AChE) in oyster gills. The results show the presence of Cu, Fe, Mn, naphthalene, benz[a]anthracene, pyrene, benz[a]pyrene and benzo[k]fluoranthene. On the other hand, AChE activity was not inhibited, which suggests that organophosphorus pollutants or carbamates were absent. Regarding oxidative stress, oysters from the estuary had oxidative damage in lipids, not proteins, and altered antioxidant enzyme activity, when compared to control organisms. Interestingly, we did not observe any correlation between the pollutants and the oxidative stress parameters evaluated in this study. Thus, we cannot rule out that a synergistic effect between the environmental variables and the pollutants is causing the oxidative stress in these oysters.