Modulation of lipase B from Candida antarctica properties via covalent immobilization on eco-friendly support for enzymatic kinetic resolution of rac-indanyl acetate

• Published in: Bioprocess and biosystems engineering Vol. 43; no. 12; pp. 2253 - 2268
• Main Authors: de Souza, Ticiane C., de Sousa Fonseca, Thiago, de Sousa Silva, Jouciane, Lima, Paula J. M., Neto, Carlos A. C. G., Monteiro, Rodolpho R. C., Rocha, Maria Valderez P., de Mattos, Marcos C., dos Santos, José C. S., Gonçalves, Luciana R. B.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2020; Springer Nature B.V
• Subjects: Acetic acid; Anacardiaceae; Bagasse; Biocatalysts; Biotechnology; Candida antarctica; Chemistry; Chemistry and Materials Science; Conversion; Environmental Engineering/Biotechnology; Enzymes; Ethylenediamine; Food Science; Glutaraldehyde; Hydrolysis; Immobilization; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Ionic strength; Lipase; Modulation; Organic solvents; Research Paper; Substrates; CALB; Enzymatic kinetic resolution; Eco-friendly support; Modulation of lipase
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-020-02411-8

In this study, the modulation of enzymatic biocatalysts were developed by the use of lipase B from Candida antarctica covalently immobilized on an eco-friendly support, cashew apple bagasse, activated with 10% glycidol–ethylenediamine–glutaraldehyde (GEG) under different immobilization strategies (5 mM or 100 mM ionic strength and in absence or presence of 0.5% (v/v) Triton X-100). The biocatalysts were characterized for thermal and organic solvents stabilities and compared with the soluble enzyme. The biocatalysts were then applied to the hydrolysis of the rac -indanyl acetate (2:1 ratio enzyme/substrate) at pH 7.0 and 30 °C for 24 h. For all the strategies evaluated, GEG promoted kinetic resolution of rac -indanyl acetate with maximum conversion (50%) and led to ( R )-indanol with excellent enantiomeric excess (97%), maintaining the maximum conversion for five consecutive cycles of hydrolysis. Therefore, the use of cashew apple bagasse has proved to be a promising eco-friendly support for enzyme immobilization, since it resulted in stable biocatalysts for enzymatic kinetic resolution. Graphic abstract