On-line packed column supercritical fluid chromatography–microwave-induced plasma atomic emission

• Published in: Journal of Chromatography A Vol. 910; no. 1; pp. 127 - 135
• Main Authors: Bertoncini, Fabrice, Thiébaut, Didier, Caude, Marcel, Gagean, Mathias, Carrazé, Bernadette, Beurdouche, Pascal, Duteurtre, Xavier
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 23.02.2001; Elsevier
• Subjects: Analytical chemistry; Carbon Dioxide - chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Gas - methods; Electron Probe Microanalysis - methods; Exact sciences and technology; Herbicides - analysis; Microwaves; Other chromatographic methods; Phenylurea Compounds - analysis; Phenylureas; Sensitivity and Specificity; Atomic emission detection; Detection, SFC; Microwave-induced plasma; Phenylureas; Trace analysis; Chemical analysis; Flow rate; Coupled method; Pesticides; Diuron; Pressure gradient; Herbicide; Supercritical fluid chromatography; Operating conditions; Pollutant; Detection limit; Ureas; Microwave induced plasma spectrometry; Detection; Atomic emission spectroscopy; Packed column; Interface
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(00)01121-3

Interfacing and evaluation of packed column supercritical fluid chromatography (SFC)–microwave-induced plasma atomic emission detection (AED) is described. Via a flow splitter and an integral restrictor, efficient transfer of solutes from column to detector without band broadening is obtained. Variation of CO 2 flow-rate during pressure gradients has little influence on both AED signal and baseline drift while it provides similar sensitivity as in capillary SFC. Continuous introduction of CO 2 in the plasma reduces the available range of emission domains; nevertheless the region of detection which is free of CO 2 interferences allows selective detection of Cl and Br as reported in this paper.