Nanocapsulated quercetin downregulates rat hepatic MMP-13 and controls diethylnitrosamine-induced carcinoma

• Published in: Nanomedicine (London, England) Vol. 9; no. 15; pp. 2323 - 2337
• Main Authors: Mandal, Ardhendu Kumar, Ghosh, Debasree, Sarkar, Sibani, Ghosh, Aparajita, Swarnakar, Snehasikta, Das, Nirmalendu
• Format: Journal Article
• Language: English
• Published: London Future Medicine Ltd 01.10.2014; Future Medicine
• Subjects: Animals; Biological and medical sciences; Cross-disciplinary physics: materials science; rheology; diethyl nitrosamine; Diethylnitrosamine - toxicity; Down-Regulation - drug effects; Exact sciences and technology; Gastroenterology. Liver. Pancreas. Abdomen; hepatocarcinogenesis; Liver - drug effects; Liver - enzymology; Liver Neoplasms, Experimental - chemically induced; Liver Neoplasms, Experimental - prevention & control; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Male; Materials science; Matrix Metalloproteinase 13 - metabolism; Medical sciences; Microscopy, Atomic Force; Microscopy, Electron, Transmission; MMP-13; Multiple tumors. Solid tumors. Tumors in childhood (general aspects); nanocapsulated quercetin; Nanocapsules; Nanoscale materials and structures: fabrication and characterization; Physics; proinflammatory cytokines; Quercetin - pharmacology; Rats; reactive oxygen species; Tumors; Oxidative stress; Reactive oxygen species; nanocapsulated quercetin; Metalloendopeptidases; Hepatic disease; Hepatocellular carcinoma; Drug carrier; hepatocarcinogenesis; Flavonoid; Carcinogenesis; Prevention; Liver cancer; Carcinogens; Polyphenols; MMP-13; Nanomedicine; Nanocapsule; proinflammatory cytokines; Enzyme; diethyl nitrosamine; Nitrosamine; Cytokine; Rats; Inflammation; Collagenase 3; Peptidases; Animals; Quercetin; Phenols; Hydrolases; Digestive diseases; Cancer; reactive oxygen species
• ISSN: 1743-5889; 1748-6963
• DOI: 10.2217/nnm.14.11

The aims of our work were to investigate the controlling role and the efficacy of nanocapsulated quercetin drug delivery system on the decrement of inflammatory mediators such as MMP-13 in diethyl nitrosamine (DEN)-induced hepatocarcinogenesis. Hepatocellular carcinoma was developed in the Swiss albino rats by the exposure of DEN. DEN administration caused the generation of reactive oxygen species, upregulation of TNF-α, IL-6, activation of MMP-13, severe oxidative damage, hyperplastic nodules with preneoplastic lesions and the histopathological changes in rat liver. Nanocapsulated quercetin treatment restricted all alterations in DEN-mediated development of hepatocarcinogenesis. Therefore, it may be concluded that nanocapsulated quercetin may be accepted as a potent therapeutic formulation in preventing DEN-mediated hepatocarcinogenesis. Original submitted 4 January 2013; Revised submitted 2 December 2013