A sensitive method for the measurement of the novel pet endectocide, selamectin (UK-124,114), in dog and cat plasma by chemical derivatisation and high-performance liquid chromatography with fluorescence detection

An analytical method has been developed for the novel pet endectocide, selamectin (USAN, UK-124, 114), in dog and cat plasma to facilitate pharmacokinetic profiling for this compound. The method involves solid phase extraction of the compound and internal standard from plasma followed by chemical de...

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Bibliographic Details
Published inJournal of pharmaceutical and biomedical analysis Vol. 24; no. 1; pp. 105 - 111
Main Authors Walker, D.K, Fenner, K.S
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.12.2000
Elsevier Science
Subjects
Analysis
Animals
Antiparasitic Agents - blood
Biological and medical sciences
Cats
Chemical derivatisation
Chromatography, High Pressure Liquid
Dogs
Drug Stability
Fluorescence
General pharmacology
High-performance liquid chromatography
Ivermectin - analogs & derivatives
Ivermectin - blood
Ivermectin - chemistry
Medical sciences
Pet endectocide
Pharmacology. Drug treatments
Selamectin
Sensitivity and Specificity
Pet endectocide
Selamectin
High-performance liquid chromatography
Chemical derivatisation
Validation
Fissipedia
Carnivora
Fluorescence detector
HPLC chromatography
Derivatization
Blood plasma
Vertebrata
Reversed phase chromatography
Mammalia
Animal
Parasiticid
Cat
Dog
Quantitative analysis
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Summary:An analytical method has been developed for the novel pet endectocide, selamectin (USAN, UK-124, 114), in dog and cat plasma to facilitate pharmacokinetic profiling for this compound. The method involves solid phase extraction of the compound and internal standard from plasma followed by chemical derivatisation using triethylamine and trifluoroacetic anhydride. This reaction yields a highly fluorescent product and thus provides a sensitive assay. Using a sample volume of 1.0 ml for dog plasma the assay has been validated over a concentration range of 0.2–40 ng/ml. Due to smaller plasma volumes for cat plasma samples, the assay was validated over a concentration range of 1.0–200 ng/ml using a sample volume of 0.2 ml. The analyte has been shown to be stable for 48 h at room temperature and through three freeze–thaw cycles in dog plasma. The analytical method is highly specific and proved suitable for the analysis of selamectin in dog and cat plasma samples following doses of compound by parenteral and non-parenteral routes.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(00)00415-5