Complementary Powerful Techniques for Investigating the Interactions of Proteins with Porous TiO2 and Its Hybrid Materials: A Tutorial Review

• Published in: Membranes (Basel) Vol. 12; no. 4; p. 415
• Main Authors: Dong, Yihui, Lin, Weifeng, Laaksonen, Aatto, Ji, Xiaoyan
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 11.04.2022; MDPI
• Subjects: Adsorption; Atomic force microscopy; atomic force microscopy (AFM); Biocompatibility; Biomedical materials; Chromatography; Energiteknik; Energy Engineering; High performance liquid chromatography; high-performance liquid chromatography (HPLC); Life sciences; Liquid chromatography; Microscopy; Molecular dynamics; molecular dynamics (MD) simulations; Pore size; Porous materials; Proteins; Raman spectra; Retention; Review; Reviews; Simulation; Structural stability; surface enhanced Raman scattering (SERS); TiO2; Titanium; Titanium dioxide; Transplants & implants
• ISSN: 2077-0375; 2077-0375
• DOI: 10.3390/membranes12040415

Understanding the adsorption and interaction between porous materials and protein is of great importance in biomedical and interface sciences. Among the studied porous materials, TiO2 and its hybrid materials, featuring distinct, well-defined pore sizes, structural stability and excellent biocompatibility, are widely used. In this review, the use of four powerful, synergetic and complementary techniques to study protein-TiO2-based porous materials interactions at different scales is summarized, including high-performance liquid chromatography (HPLC), atomic force microscopy (AFM), surface-enhanced Raman scattering (SERS), and Molecular Dynamics (MD) simulations. We expect that this review could be helpful in optimizing the commonly used techniques to characterize the interfacial behavior of protein on porous TiO2 materials in different applications.