Expression Profile of Genes Encoding Proteins Involved in Regulation of Vasculature Development and Heart Muscle Morphogenesis—A Transcriptomic Approach Based on a Porcine Model
Despite significant advances in treatment of acute coronary syndromes (ACS) many subjects still develop heart failure due to significantly reduced ejection fraction. Currently, there are no commonly available treatment strategies that replace the infarcted/dysfunctional myocardium. Therefore, unders...
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| Published in | International journal of molecular sciences Vol. 22; no. 16; p. 8794 |
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| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Basel
MDPI AG
16.08.2021
MDPI |
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| Online Access | Get full text |
| ISSN | 1422-0067 1661-6596 1422-0067 |
| DOI | 10.3390/ijms22168794 |
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| Abstract | Despite significant advances in treatment of acute coronary syndromes (ACS) many subjects still develop heart failure due to significantly reduced ejection fraction. Currently, there are no commonly available treatment strategies that replace the infarcted/dysfunctional myocardium. Therefore, understanding the mechanisms that control the regeneration of the heart muscle is important. The development of new coronary vessels plays a pivotal role in cardiac regeneration. Employing microarray expression assays and RT-qPCR validation expression pattern of genes in long-term primary cultured cells isolated form the right atrial appendage (RAA) and right atrium (RA) was evaluated. After using DAVID software, it indicated the analysis expression profiles of genes involved in ontological groups such as: “angiogenesis”, “blood vessel morphogenesis”, “circulatory system development”, “regulation of vasculature development”, and “vasculature development” associated with the process of creation new blood vessels. The performed transcriptomic comparative analysis between two different compartments of the heart muscle allowed us to indicate the presence of differences in the expression of key transcripts depending on the cell source. Increases in culture intervals significantly increased expression of SFRP2, PRRX1 genes and some other genes involved in inflammatory process, such as: CCL2, IL6, and ROBO1. Moreover, the right atrial appendage gene encoding lysyl oxidase (LOX) showed much higher expression compared to the pre-cultivation state. |
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| AbstractList | Despite significant advances in treatment of acute coronary syndromes (ACS) many subjects still develop heart failure due to significantly reduced ejection fraction. Currently, there are no commonly available treatment strategies that replace the infarcted/dysfunctional myocardium. Therefore, understanding the mechanisms that control the regeneration of the heart muscle is important. The development of new coronary vessels plays a pivotal role in cardiac regeneration. Employing microarray expression assays and RT-qPCR validation expression pattern of genes in long-term primary cultured cells isolated form the right atrial appendage (RAA) and right atrium (RA) was evaluated. After using DAVID software, it indicated the analysis expression profiles of genes involved in ontological groups such as: "angiogenesis", "blood vessel morphogenesis", "circulatory system development", "regulation of vasculature development", and "vasculature development" associated with the process of creation new blood vessels. The performed transcriptomic comparative analysis between two different compartments of the heart muscle allowed us to indicate the presence of differences in the expression of key transcripts depending on the cell source. Increases in culture intervals significantly increased expression of SFRP2, PRRX1 genes and some other genes involved in inflammatory process, such as: CCL2, IL6, and ROBO1. Moreover, the right atrial appendage gene encoding lysyl oxidase (LOX) showed much higher expression compared to the pre-cultivation state.Despite significant advances in treatment of acute coronary syndromes (ACS) many subjects still develop heart failure due to significantly reduced ejection fraction. Currently, there are no commonly available treatment strategies that replace the infarcted/dysfunctional myocardium. Therefore, understanding the mechanisms that control the regeneration of the heart muscle is important. The development of new coronary vessels plays a pivotal role in cardiac regeneration. Employing microarray expression assays and RT-qPCR validation expression pattern of genes in long-term primary cultured cells isolated form the right atrial appendage (RAA) and right atrium (RA) was evaluated. After using DAVID software, it indicated the analysis expression profiles of genes involved in ontological groups such as: "angiogenesis", "blood vessel morphogenesis", "circulatory system development", "regulation of vasculature development", and "vasculature development" associated with the process of creation new blood vessels. The performed transcriptomic comparative analysis between two different compartments of the heart muscle allowed us to indicate the presence of differences in the expression of key transcripts depending on the cell source. Increases in culture intervals significantly increased expression of SFRP2, PRRX1 genes and some other genes involved in inflammatory process, such as: CCL2, IL6, and ROBO1. Moreover, the right atrial appendage gene encoding lysyl oxidase (LOX) showed much higher expression compared to the pre-cultivation state. Despite significant advances in treatment of acute coronary syndromes (ACS) many subjects still develop heart failure due to significantly reduced ejection fraction. Currently, there are no commonly available treatment strategies that replace the infarcted/dysfunctional myocardium. Therefore, understanding the mechanisms that control the regeneration of the heart muscle is important. The development of new coronary vessels plays a pivotal role in cardiac regeneration. Employing microarray expression assays and RT-qPCR validation expression pattern of genes in long-term primary cultured cells isolated form the right atrial appendage (RAA) and right atrium (RA) was evaluated. After using DAVID software, it indicated the analysis expression profiles of genes involved in ontological groups such as: “angiogenesis”, “blood vessel morphogenesis”, “circulatory system development”, “regulation of vasculature development”, and “vasculature development” associated with the process of creation new blood vessels. The performed transcriptomic comparative analysis between two different compartments of the heart muscle allowed us to indicate the presence of differences in the expression of key transcripts depending on the cell source. Increases in culture intervals significantly increased expression of SFRP2, PRRX1 genes and some other genes involved in inflammatory process, such as: CCL2, IL6, and ROBO1. Moreover, the right atrial appendage gene encoding lysyl oxidase (LOX) showed much higher expression compared to the pre-cultivation state. |
| Author | Mozdziak, Paul Jemielity, Marek Jopek, Karol Kempisty, Bartosz Nawrocki, Mariusz J. Zdun, Maciej Perek, Bartłomiej Bukowska, Dorota |
| AuthorAffiliation | 3 Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland; maciejzdun@umk.pl 4 Physiology Graduate Program, North Carolina State University, Raleigh, NC 27695, USA; pemozdzi@ncsu.edu 2 Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznań, Poland; karoljopek@ump.edu.pl 5 Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USA 1 Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland; mjnawrocki@ump.edu.pl 6 Department of Cardiac Surgery and Transplantology, Poznan University of Medical Sciences, 61-848 Poznań, Poland; kardiock@ump.edu.pl (M.J.); bperek@ump.edu.pl (B.P.) 8 Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland 7 Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus |
| AuthorAffiliation_xml | – name: 8 Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland – name: 7 Department of Diagnostics and Clinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland; dbukowska@umk.pl – name: 6 Department of Cardiac Surgery and Transplantology, Poznan University of Medical Sciences, 61-848 Poznań, Poland; kardiock@ump.edu.pl (M.J.); bperek@ump.edu.pl (B.P.) – name: 1 Department of Anatomy, Poznan University of Medical Sciences, 60-781 Poznań, Poland; mjnawrocki@ump.edu.pl – name: 5 Prestage Department of Poultry Science, North Carolina State University, Raleigh, NC 27695, USA – name: 4 Physiology Graduate Program, North Carolina State University, Raleigh, NC 27695, USA; pemozdzi@ncsu.edu – name: 2 Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznań, Poland; karoljopek@ump.edu.pl – name: 3 Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland; maciejzdun@umk.pl |
| Author_xml | – sequence: 1 givenname: Mariusz J. orcidid: 0000-0003-3753-755X surname: Nawrocki fullname: Nawrocki, Mariusz J. – sequence: 2 givenname: Karol orcidid: 0000-0002-7399-0303 surname: Jopek fullname: Jopek, Karol – sequence: 3 givenname: Maciej orcidid: 0000-0002-1126-1084 surname: Zdun fullname: Zdun, Maciej – sequence: 4 givenname: Paul orcidid: 0000-0002-1575-3123 surname: Mozdziak fullname: Mozdziak, Paul – sequence: 5 givenname: Marek surname: Jemielity fullname: Jemielity, Marek – sequence: 6 givenname: Bartłomiej orcidid: 0000-0003-2398-9571 surname: Perek fullname: Perek, Bartłomiej – sequence: 7 givenname: Dorota surname: Bukowska fullname: Bukowska, Dorota – sequence: 8 givenname: Bartosz surname: Kempisty fullname: Kempisty, Bartosz |
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