Calcium citrate： a new biomaterial that can enhance bone formation in situ

• Published in: Chinese journal of traumatology Vol. 15; no. 5; pp. 291 - 296
• Main Authors: Li-ming, Wang, Wei, Wang, Xiu-cui, Li, Lei, Peng, Zhong-qin, Lin, Hua-zi, Xü
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.10.2012; Department of Orthopaedic Surgery,Affiliated Hospital of Binzhou Medical College,Binzhou 256603,Shandong Province,China%Department of Orthopaedic Surgery,Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China%Department of Respiratory Medicine,Yuying Children's Hospital Affiliated to Wenzhou Medical College,Wenzhou 325000,China%Department of Orthopaedic Surgery,Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,China; Trauma Center of the Affiliated Hospital of Hainan Medical College,Haikou 571100,China%Hospital of Integrated Traditional Chinese and Western Medicine of Wenzhou City,Wenzhou 325000,China; Elsevier
• Subjects: Animals; Biocompatible Materials; Bone morphogenetic protein-2; Bone Regeneration; Calcium Citrate; Gelatin; Humans; Osteogenesis; rhBMP-2; 原位; 柠檬酸钙; 生物材料; 随机平均; 骨形态发生蛋白-2; 骨形成; 骨缺损; Bone morphogenetic protein-2; Gelatin; Calcium citrate; Biocompatible materials
• ISSN: 1008-1275
• DOI: 10.3760/cma.j.issn.1008-1275.2012.05.007

Objective： To investigate the effect of a new biomaterial combining calcium citrate and recombinant human bone morphogenetic protein-2 （rhBMP-2） on bone regeneration in a bone defect rabbit model. Methods： Totally 30 male New Zealand white rabbits were randomly and equally divided into calcium citraterhBMP-2 （CC-rhBMP-2） group and rhBMP-2 only group. Two 10 ram-long and 5 ram-deep bone defects were respectively created in the left and right femoral condyles of the rabbits. Subsequently 5 pellets of calcium citrate （10 mg） combined with rhBMP-2 （2 rag） or rhBMP-2 alone were implanted into the bone defects and compressed with cotton swab. Bone granules were obtained at 2, 4 and 6 weeks after procedure and received histological analysis. LSD t-test and a subsequent t-test were adopted for statistical analysis. Results： Histomorphometric analysis revealed newlyformed bones, and calcium citrate has been absorbed in the treatment group. The percent of newly formed bone area in femoral condyle in control group and CC-rhBMP-2 group was respectively 31.73%±1.26% vs 48.21%±2.37% at 2 weeks; 43.40%±1.65% vs 57.32%±1.47% at 4 weeks, and 51.32%±7.80% vs 66.74%±4.05% at 6 weeks （P〈0.05 for all）. At 2 weeks, mature cancellous bone was observed to be already formed in the treatment group. Conclusion： From this study, it can be concluded that calcium citrate combined with rhBMP-2 signifcantly enhances bone regeneration in bone defects. This synthetic gelatin matrix stimulates formation of new bone and bone marrow in the defect areas by releasing calcium ions.