Mucins and CD56 as markers of tumour invasion and prognosis in periampullary cancer

• Published in: British journal of surgery Vol. 97; no. 8; pp. 1269 - 1278
• Main Authors: Aloysius, M. M., Zaitoun, A. M., Awad, S., Ilyas, M., Rowlands, B. J., Lobo, D. N.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.08.2010; Wiley
• Subjects: Aged; Ampulla of Vater - pathology; Biological and medical sciences; Biomarkers, Tumor - metabolism; CD56 Antigen - metabolism; Common Bile Duct Neoplasms - diagnosis; Common Bile Duct Neoplasms - mortality; Common Bile Duct Neoplasms - pathology; Female; General aspects; Humans; Immunohistochemistry; Kaplan-Meier Estimate; Male; Medical sciences; Middle Aged; Mucins - metabolism; Neoplasm Invasiveness; Pancreatitis, Chronic - diagnosis; Prognosis; Prognosis; Neural cell adhesion molecule; Biological marker; Biological indicator; Tumorous infiltration; Metastasis; Malignant tumor; Invasion; Natural killer cell; Medicine; Mucin; Treatment; Surgery; Periampullary; Cancer
• ISSN: 0007-1323; 1365-2168
• DOI: 10.1002/bjs.7107

Background: This study investigated the association of mucins and cluster of differentiation (CD) 56 with vascular and perineural invasion and survival in patients with periampullary cancer. Methods: Immunohistochemical staining was performed on formalin‐fixed pancreatic tissue microarrays (cancer, chronic pancreatitis and normal pancreatic tissue) constructed from 126 pancreatic resections (cancer, 104; chronic pancreatitis, 22). Mucin (MUC) 1, MUC4 and MUC5AC expression was quantified using the immunohistochemical score (range 0–300), MUC3 expression was described as membranous or cytoplasmic, and expression of CD56, MUC2 and MUC6 as present or absent. Results: In cancers, vascular invasion correlated with overexpression (immunohistochemical score of 100 or more) of MUC1 (P = 0·003) and presence of MUC6 (P = 0·024), and perineural invasion correlated with overexpression of MUC5AC (P = 0·015). Reduced survival was observed with overexpression of MUC4 (P = 0·032) and MUC5AC (P = 0·048), membranous expression of MUC3 (P = 0·048), and presence of CD56 (P = 0·041). Perineural invasion also correlated with CD56 expression (P = 0·001). Overexpression of MUC4 and MUC5AC correlated with tumour recurrence (P = 0·001 and P = 0·034 respectively). Multivariable analysis identified membranous expression of MUC3 (P = 0·023), lymphatic invasion (P = 0·015) and perineural invasion (P = 0·004) as independent predictors of poor survival. Conclusion: Mucins and CD56 may be markers of prognosis in patients with periampullary cancer. Copyright © 2010 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd. Promising predictors of prognosis