High-Intensity Red Light-Emitting Diode Irradiation Suppresses the Inflammatory Response of Human Periodontal Ligament Stem Cells by Promoting Intracellular ATP Synthesis

• Published in: Life (Basel, Switzerland) Vol. 12; no. 5; p. 736
• Main Authors: Yamauchi, Nobuhiro, Minagawa, Emika, Imai, Kazutaka, Kobuchi, Kenjiro, Li, Runbo, Taguchi, Yoichiro, Umeda, Makoto
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 15.05.2022; MDPI
• Subjects: Adenosine triphosphate; ATP; Bone marrow; Cell culture; Cytochrome; Cytokines; Cytotoxicity; Inflammation; Inflammatory response; Interleukin 6; Interleukin 8; Irradiance; Irradiation; Ligaments; Light emitting diodes; Light sources; Luminous intensity; Medical ethics; Medical research; Periodontal ligament; periodontal ligament stem cells; Periodontitis; photobiomodulation therapy; Potassium cyanide; Regeneration (physiology); Stem cells; Synthesis; Tissue engineering; Tumor necrosis factor-TNF; Tumor necrosis factor-α; United States > US; Japan; photobiomodulation therapy; periodontal ligament stem cells; adenosine triphosphate
• ISSN: 2075-1729; 2075-1729
• DOI: 10.3390/life12050736

Periodontitis is an inflammatory lesion in the periodontal tissue. The behavior of human periodontal ligament stem cells (hPDLSCs), which play an important role in periodontal tissue regeneration, is restricted by the influence of inflammatory mediators. Photobiomodulation therapy exerts anti-inflammatory effects. The purpose of this study was to investigate the effects of light-emitting diode (LED) irradiation on the inflammatory responses of hPDLSCs. The light source was a red LED (peak wavelength: 650 nm), and the total absolute irradiance was 400 mW/cm . The inflammatory response in hPDLSCs is induced by tumor necrosis factor (TNF)-α. Adenosine triphosphate (ATP) levels and pro-inflammatory cytokine (interleukin [IL]-6 and IL-8) production were measured 24 h after LED irradiation, and the effects of potassium cyanide (KCN) were investigated. LED irradiation at 6 J/cm significantly increased the ATP levels and reduced TNF-α-induced IL-6 and IL-8 production. Furthermore, the inhibitory effect of LED irradiation on the production of pro-inflammatory cytokines was inhibited by KCN treatment. The results of this study showed that high-intensity red LED irradiation suppressed the TNF-α-stimulated pro-inflammatory cytokine production in hPDLSCs by promoting ATP synthesis. These results suggest that high-intensity red LED is a useful tool for periodontal tissue regeneration in chronically inflamed tissues.