Origin of the murine implantation serine proteinase subfamily

• Published in: Molecular reproduction and development Vol. 69; no. 2; pp. 126 - 136
• Main Authors: O'Sullivan, Colleen M., Tang, Lin, Xu, Hui, Liu, Shiying, Rancourt, Derrick E.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.10.2004; Wiley-Liss
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Embryology: invertebrates and vertebrates. Teratology; Exons; Fundamental and applied biological sciences. Psychology; gene evolution; gene family; gene regulation; gene structure; Genomic Library; Immunohistochemistry; implantation; In Situ Hybridization; Introns; Mice; Molecular embryology; Molecular Sequence Data; Regulatory Sequences, Nucleic Acid; Sequence Alignment; Serine Endopeptidases - genetics; serine proteinases; Tryptases; Serine endopeptidases; Enzyme; Rodentia; Sequence alignment; Implantation; Gene organization; Implantation Serine Proteinase; Serine enzyme; Molecular evolution; Peptidases; Vertebrata; Mammalia; Mouse; Blastocyte; Tryptase; Hydrolases; Regulatory sequence; Molecular cloning
• ISSN: 1040-452X; 1098-2795
• DOI: 10.1002/mrd.20115

The S1 serine protease family is one of the largest gene families known. Within this family there are several subfamilies that have been grouped together as a result of sequence comparisons and substrate identification. The grouping of related genes allows for the speculation of function for newly found members by comparison and for novel subfamilies by contrast. Analysis of the evolutionary patterns of genes indicates whether or not orthologs are likely to be identified in other species as well as potentially indicating that hypothesized orthologs are in fact not. Looking at subtle differences between subfamily members can reveal intricacies about function and expression. Previously, we have described genes encoding two novel serine proteinases, ISP1 and ISP2, which are most closely related to tryptases. The ISP1 gene encodes the embryo‐derived enzyme strypsin, which is necessary for blastocyst hatching and invasion in vitro. Additionally both ISP1 and ISP2 are co‐expressed in the endometrial gland during the time of hatching, suggesting that they may also both participate in zona lysis from within the uterine lumen. Here, we demonstrate that the ISPs are tandemly linked within the tryptase cluster on 17A3.3. We suggest that remarkable similarities within the 5′‐untranslated and first intron regions of ISP1 and ISP2 may explain their intimate co‐regulation in uterus. We also suggest that ISP genes have evolved through gene duplication and that the ISP1 gene has also begun to adopt an additional new function in the murine preimplantation embryo. Mol. Reprod. Dev. 69: 126–136, 2004. © 2004 Wiley‐Liss, Inc.