Expression of CD11/CD18 and ICAM-1 on monocytes and lymphocytes of HIV-1-infected individuals

• Published in: Journal of leukocyte biology Vol. 56; no. 3; pp. 304 - 309
• Main Authors: Stent, Gianna, Cameron, Paul U., Crowe, Suzanne M.
• Format: Journal Article
• Language: English
• Published: United States Society for Leukocyte Biology 01.09.1994
• Subjects: Acquired Immunodeficiency Syndrome - metabolism; Acquired Immunodeficiency Syndrome - pathology; Acquired Immunodeficiency Syndrome - physiopathology; Antigens, CD - analysis; Antigens, CD - metabolism; Antigens, CD - physiology; CD11 Antigens; CD4; CD8 Antigens - analysis; CD8 Antigens - metabolism; CD8 Antigens - physiology; Cell Adhesion Molecules - analysis; Cell Adhesion Molecules - metabolism; Cell Adhesion Molecules - physiology; disease progression; dual labeling; Flow Cytometry; HIV-1 - isolation & purification; human immunodeficiency virus 1; Humans; Intercellular Adhesion Molecule-1; Monocytes - chemistry; Monocytes - microbiology; Monocytes - pathology
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/jlb.56.3.304

The role of the leukocyte integrins in the infection of cells by HIV‐1 or in the progression of AIDS‐related disease is of continuing interest. Using a duallabeling flow cytometric method, we determined the level of expression of the leukocyte integrins and intercellular adhesion molecule 1 (ICAM‐1) on monocytes and lymphocytes from HIV‐1‐infected and uninfected individuals. The mean fluorescence of CD18 on lymphocytes and CD11c on monocytes of HIV‐1‐infected subjects was significantly higher than for the control group (P=.008 and.014, respectively). There was a trend toward higher fluorescence of CD11a on lymphocytes from HIV‐1‐infected subjects compared with cells from the control group (P=.089). Integrin expression on lymphocytes or monocytes from HIV‐infected individuals did not correlate with their CD4 lymphocyte number. However, the mean fluorescence associated with ICAM‐1 on monocytes and CD11a and CD18 expression on lymphocytes was related to clinical stage of disease. J. Leukoc. Biol. 56: 304–309; 1994.