Insulin-like growth factor 1-coated sutures improve anastomotic healing in an experimental model of colitis

Background: Exogenously applied insulin‐like growth factor (rhIGF‐1) may improve normal intestinal healing. This study examined the effect of rhIGF‐1‐coated sutures on anastomotic healing in experimental colitis. Methods: Acute colitis was induced in rats by dextran sodium sulphate (DSS). Inflammati...

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Published inBritish journal of surgery Vol. 97; no. 2; pp. 258 - 265
Main Authors Rijcken, E., Fuchs, T., Sachs, L., Kersting, C. M., Bruewer, M., Krieglstein, C. F.
Format Journal Article
LanguageEnglish
Published Chichester, UK John Wiley & Sons, Ltd 01.02.2010
Wiley
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Summary:Background: Exogenously applied insulin‐like growth factor (rhIGF‐1) may improve normal intestinal healing. This study examined the effect of rhIGF‐1‐coated sutures on anastomotic healing in experimental colitis. Methods: Acute colitis was induced in rats by dextran sodium sulphate (DSS). Inflammation was assessed by clinical Disease Activity Index (DAI), myeloperoxidase (MPO) measurement and histological examination. A distal colonic anastomosis was performed using sutures coated with rhIGF‐1 dissolved in poly(D,L‐lactide) (PDLLA) under general anaesthetic. Anastomotic healing was evaluated histologically, and by hydroxyproline measurement and bursting parameters after 1, 3 and 7 days, and compared with healthy, DSS and DSS + PDLLA controls. Results: DAI, MPO and histological inflammation scores were significantly increased in all animals treated with DSS. Bursting occurred less often within the anastomotic line on day 3 in the IGF group than in DSS controls (three versus eight of ten). On day 7, the IGF group had significantly increased histological healing scores (mean(s.e.m.) 12·5(0·7) versus 9·2(0·8) (P < 0·050)) and hydroxyproline content (4·6(0·3) versus 3·6(0·1) mg/g tissue; P < 0·050) compared with DSS controls. Conclusion: IGF‐1‐coated sutures improve important aspects of anastomotic healing in rats with experimental colitis. Copyright © 2010 British Journal of Surgery Society Ltd. Published by John Wiley & Sons, Ltd. Biosutures may advance healing
Bibliography:ark:/67375/WNG-ZR4JC72H-S
istex:FDA18D0C489AE84754E885D9B22D7E350EB44698
'Innovative Medical Research' of the University of Muenster Medical School - No. KR 2 2 04 05
ArticleID:BJS6781
Presented in part to the 42nd Congress of the European Society for Surgical Research, Rotterdam, The Netherlands, May 2007, and to the 11th Surgical Research Days, Saarbruecken, Germany, November 2007 and published in abstract form as Eur Surg Res 2007; 39(Suppl. 1): 1-90, O27, and Langenbecks Arch Surg 2007; 392: 783-A31.
Eur Surg Res
2007
39
Presented in part to the 42nd Congress of the European Society for Surgical Research, Rotterdam, The Netherlands, May 2007, and to the 11th Surgical Research Days, Saarbruecken, Germany, November 2007 and published in abstract form as
Langenbecks Arch Surg
392
(Suppl. 1): 1–90, O27, and
783–A31.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0007-1323
1365-2168
DOI:10.1002/bjs.6781