Xyloglucan Fucosylation Modulates Arabidopsis Cell Wall Hemicellulose Aluminium binding Capacity
Although xyloglucan (XyG) is reported to bind Aluminium (Al), the influence of XyG fucosylation on the cell wall Al binding capacity and plant Al stress responses is unclear. We show that Arabidopsis T-DNA insertion mutants with reduced AXY3 ( XYLOSIDASE1 ) function and consequent reduced levels of...
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Published in | Scientific reports Vol. 8; no. 1; p. 428 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
11.01.2018
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Although xyloglucan (XyG) is reported to bind Aluminium (Al), the influence of XyG fucosylation on the cell wall Al binding capacity and plant Al stress responses is unclear. We show that
Arabidopsis
T-DNA insertion mutants with reduced
AXY3
(
XYLOSIDASE1
) function and consequent reduced levels of fucosylated XyG are more sensitive to Al than wild-type Col-0 (WT). In contrast, T-DNA insertion mutants with reduced
AXY8
(
FUC95A
) function and consequent increased levels of fucosylated XyG are more Al resistant.
AXY3
transcript levels are strongly down regulated in response to 30 min Al treatment, whilst
AXY8
transcript levels also repressed until 6 h following treatment onset. Mutants lacking
AXY3
or
AXY8
function exhibit opposing effects on Al contents of root cell wall and cell wall hemicellulose components. However, there was no difference in the amount of Al retained in the pectin components between mutants and WT. Finally, whilst the total sugar content of the hemicellulose fraction did not change, the altered hemicellulose Al content of the mutants is shown to be a likely consequence of their different XyG fucosylation levels. We conclude that variation in XyG fucosylation levels influences the Al sensitivity of
Arabidopsis
by affecting the Al-binding capacity of hemicellulose. |
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-017-18711-1 |