5-Hydroxymethylfurfural from Black Garlic Extract Prevents TNFα-induced Monocytic Cell Adhesion to HUVECs by Suppression of Vascular Cell Adhesion Molecule-1 Expression, Reactive Oxygen Species Generation and NF-κB Activation

• Published in: Phytotherapy research Vol. 25; no. 7; pp. 965 - 974
• Main Authors: Kim, Hye Kyung, Choi, Young-Whan, Lee, Eun Na, Park, Jin Kyeong, Kim, Sun-Gun, Park, Da-Jung, Kim, Bong-Seon, Lim, Young-Tak, Yoon, Sik
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.07.2011; Wiley
• Subjects: 5-hydroxymethylfurfural; aged black garlic; Anti-Inflammatory Agents - pharmacology; Biological and medical sciences; Cell Adhesion - drug effects; cell adhesion molecules; Cell Adhesion Molecules - antagonists & inhibitors; Cell Line; Furaldehyde - analogs & derivatives; Furaldehyde - pharmacology; Garlic - chemistry; General pharmacology; Human Umbilical Vein Endothelial Cells; Humans; Intercellular Adhesion Molecule-1 - metabolism; Medical sciences; Monocytes - drug effects; NF-kappa B - antagonists & inhibitors; NF-κB transcription factor; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Extracts - pharmacology; reactive oxygen species; Reactive Oxygen Species - antagonists & inhibitors; Reactive Oxygen Species - metabolism; Tumor Necrosis Factor-alpha - pharmacology; Vascular Cell Adhesion Molecule-1 - metabolism; Human; Endothelial cell; Reactive oxygen species; Vascular cell adhesion molecule 1; cell adhesion molecules; Monocyte; Pharmacognosy; Cytokine; Cell adhesion molecule; Furfural(hydroxymethyl); Extract; Medicinal plant; NF-kB transcription factor; aged black garlic; Cell adhesion; Plant origin; 5-hydroxymethylfurfural; human umbilical vein endothelial cells; Flavoring crop; Garlic; Transcription factor NFκB; Transcription factor; Umbilical vein; Tumor necrosis factor α
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.3351

5‐Hydroxymethylfurfural (5‐HMF) is a common Maillard reaction product; the reaction occurs during heat‐processing and the preparation of many types of foods and beverages. Although 5‐HMF has been proposed to have harmful effects, recently, its beneficial effects, including antioxidant, cytoprotective and antitumor effects have become increasingly apparent. It was found recently that a chloroform extract of aged black garlic shows antiinflammatory properties when administered to human umbilical vein endothelial cells (HUVECs). This study investigated the antiinflammatory potential of 5‐HMF purified from the chloroform extract of aged black garlic in tumor necrosis factor‐α (TNF‐α)‐stimulated HUVECs. Treatment of HUVECs with 5‐HMF strongly suppressed TNF‐α‐induced cell surface and total protein expression of vascular cell adhesion molecule‐1 (VCAM‐1) and intercellular cell adhesion molecule‐1 (ICAM‐1) as well as their mRNA expression. In addition, 5‐HMF significantly inhibited TNF‐α‐induced reactive oxygen species formation, and markedly reduced THP‐1 monocyte adhesion to TNF‐α‐stimulated HUVECs. Furthermore, 5‐HMF significantly inhibited NF‐κB transcription factor activation in TNF‐α‐stimulated HUVECs. The data provide new evidence of the antiinflammatory properties of 5‐HMF in support of its potential therapeutic use for the prevention and management of vascular diseases such as atherosclerosis through mechanisms involving the inhibition of VCAM‐1 expression and NF‐κB activation in vascular endothelial cells. Copyright © 2011 John Wiley & Sons, Ltd.